Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Jahaun Azadmanesh, Scott R. Trickel, Kevin L. Weiss, Leighton Coates, Gloria E Borgstahl

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.The growth of crystals of perdeuterated human manganese superoxide dismutase with a unit-cell edge of 240 was optimized for neutron data collection. Data were collected to 2.30 Å resolution from a 0.26 mm3 crystal. This is the crystal with the largest unit-cell edge from which data have been collected via neutron diffraction to sufficient resolution where hydrogen positions can be observed.

Original languageEnglish (US)
Pages (from-to)235-240
Number of pages6
JournalActa Crystallographica Section:F Structural Biology Communications
Volume73
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Neutron Diffraction
inorganic peroxides
Neutron diffraction
Superoxide Dismutase
neutron diffraction
manganese
Hydrogen
Crystals
Neutrons
Crystallization
crystals
hydrogen
cells
Oxidative stress
Manganese
neutrons
Superoxides
Hydrogen Peroxide
peroxides
Catalytic Domain

Keywords

  • human
  • large unit cell
  • manganese superoxide dismutase
  • neutron diffraction
  • perdeuteration

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals. / Azadmanesh, Jahaun; Trickel, Scott R.; Weiss, Kevin L.; Coates, Leighton; Borgstahl, Gloria E.

In: Acta Crystallographica Section:F Structural Biology Communications, Vol. 73, No. 4, 01.04.2017, p. 235-240.

Research output: Contribution to journalArticle

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