Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-β receptor promoter DNA

Vinod B. Agarkar, Nigar D. Babayeva, A Angie Rizzino, Tahir H Tahirov

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular pro - liferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C - terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-β receptor promoter (TβR-II) DNA. The crystals belonged to space group P212 121, with unit-cell parameters a = 42.66, b = 52, c = 99.78 Å, and diffracted to a resolution of 2.2 Å.

Original languageEnglish (US)
Pages (from-to)1261-1263
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number12
DOIs
StatePublished - Dec 1 2009

Fingerprint

mice
deoxyribonucleic acid
Proto-Oncogene Proteins c-ets
Biological Phenomena
DNA
apoptosis
Apoptosis
Gene Expression
Amino Acids
genes
amino acids
Transcription Factors
Genes
fragments
proteins
Crystals
Proteins
cells
crystals
DNA receptor

Keywords

  • Cancer
  • Elf3
  • Ets
  • Transcription factors

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

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title = "Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-β receptor promoter DNA",
abstract = "Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular pro - liferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C - terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-β receptor promoter (TβR-II) DNA. The crystals belonged to space group P212 121, with unit-cell parameters a = 42.66, b = 52, c = 99.78 {\AA}, and diffracted to a resolution of 2.2 {\AA}.",
keywords = "Cancer, Elf3, Ets, Transcription factors",
author = "Agarkar, {Vinod B.} and Babayeva, {Nigar D.} and Rizzino, {A Angie} and Tahirov, {Tahir H}",
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T1 - Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-β receptor promoter DNA

AU - Agarkar, Vinod B.

AU - Babayeva, Nigar D.

AU - Rizzino, A Angie

AU - Tahirov, Tahir H

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular pro - liferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C - terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-β receptor promoter (TβR-II) DNA. The crystals belonged to space group P212 121, with unit-cell parameters a = 42.66, b = 52, c = 99.78 Å, and diffracted to a resolution of 2.2 Å.

AB - Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular pro - liferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C - terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-β receptor promoter (TβR-II) DNA. The crystals belonged to space group P212 121, with unit-cell parameters a = 42.66, b = 52, c = 99.78 Å, and diffracted to a resolution of 2.2 Å.

KW - Cancer

KW - Elf3

KW - Ets

KW - Transcription factors

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