Predicting the Subcellular Localization of Human Proteins Using Machine Learning and Exploratory Data Analysis

George K. Acquaah-Mensah, Sonia M. Leach, Chittibabu Guda

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Identifying the subcellular localization of proteins is particularly helpful in the functional annotation of gene products. In this study, we use Machine Learning and Exploratory Data Analysis (EDA) techniques to examine and characterize amino acid sequences of human proteins localized in nine cellular compartments. A dataset of 3,749 protein sequences representing human proteins was extracted from the SWISS-PROT database. Feature vectors were created to capture specific amino acid sequence characteristics. Relative to a Support Vector Machine, a Multi-layer Perceptron, and a Naïve Bayes classifier, the C4.5 Decision Tree algorithm was the most consistent performer across all nine compartments in reliably predicting the subcellular localization of proteins based on their amino acid sequences (average Precision=0.88; average Sensitivity=0.86). Furthermore, EDA graphics characterized essential features of proteins in each compartment. As examples, proteins localized to the plasma membrane had higher proportions of hydrophobic amino acids; cytoplasmic proteins had higher proportions of neutral amino acids; and mitochondrial proteins had higher proportions of neutral amino acids and lower proportions of polar amino acids. These data showed that the C4.5 classifier and EDA tools can be effective for characterizing and predicting the subcellular localization of human proteins based on their amino acid sequences.

Original languageEnglish (US)
Pages (from-to)120-133
Number of pages14
JournalGenomics, Proteomics and Bioinformatics
Volume4
Issue number2
DOIs
Publication statusPublished - May 1 2006

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Keywords

  • Decision Tree
  • Exploratory Data Analysis
  • Machine Learning
  • subcellular localization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Computational Mathematics

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