Precise excision of bacterial transposon Tn 5 in yeast

D. A. Gordenin, M. V. Trofimova, O. N. Shaburova, Youri I Pavlov, Y. O. Chernoff, Y. V. Chekuolene, Y. Y. Proscyavichus, K. V. Sasnauskas, A. A. Janulaitis

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have demonstrated that precise excision of bacterial transposon Tn 5 can occur in the yeast, Saccharomyces cerevisiae. Tn 5 insertions in the yeast gene LYS2 were generated by transposon mutagenesis made in Escherichia coli by means of a λ::Tn 5 vector. Nine insertions of Tn 5 into the structural part of the yeast LYS2 gene situated in a shuttle epsiomal plasmid were selected. All the plasmids with a Tn 5 insertion were used to transform yeast strains carrying a deletion of the entire LYS2 gene or a deletion of the part of LYS2 overlapping the point of insertion. All insertions inactivated the LYS2 gene and were able to revert with low (about 10-8) frequencies to lysine prototrophy. Restriction analysis of revertant plasmids revealed them to be indistinguishable from the original plasmid without Tn 5 insertion. DNA sequencing of the regions containing the points of insertions, made for two revertants, proved that Tn 5 excision was completely precise.

Original languageEnglish (US)
Pages (from-to)388-393
Number of pages6
JournalMGG Molecular & General Genetics
Volume213
Issue number2-3
DOIs
StatePublished - Aug 1 1988

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Plasmids
Yeasts
Genes
DNA Sequence Analysis
Mutagenesis
Lysine
Saccharomyces cerevisiae
Escherichia coli

Keywords

  • Tn 5
  • Transposon excision
  • Yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Gordenin, D. A., Trofimova, M. V., Shaburova, O. N., Pavlov, Y. I., Chernoff, Y. O., Chekuolene, Y. V., ... Janulaitis, A. A. (1988). Precise excision of bacterial transposon Tn 5 in yeast. MGG Molecular & General Genetics, 213(2-3), 388-393. https://doi.org/10.1007/BF00339607

Precise excision of bacterial transposon Tn 5 in yeast. / Gordenin, D. A.; Trofimova, M. V.; Shaburova, O. N.; Pavlov, Youri I; Chernoff, Y. O.; Chekuolene, Y. V.; Proscyavichus, Y. Y.; Sasnauskas, K. V.; Janulaitis, A. A.

In: MGG Molecular & General Genetics, Vol. 213, No. 2-3, 01.08.1988, p. 388-393.

Research output: Contribution to journalArticle

Gordenin, DA, Trofimova, MV, Shaburova, ON, Pavlov, YI, Chernoff, YO, Chekuolene, YV, Proscyavichus, YY, Sasnauskas, KV & Janulaitis, AA 1988, 'Precise excision of bacterial transposon Tn 5 in yeast', MGG Molecular & General Genetics, vol. 213, no. 2-3, pp. 388-393. https://doi.org/10.1007/BF00339607
Gordenin DA, Trofimova MV, Shaburova ON, Pavlov YI, Chernoff YO, Chekuolene YV et al. Precise excision of bacterial transposon Tn 5 in yeast. MGG Molecular & General Genetics. 1988 Aug 1;213(2-3):388-393. https://doi.org/10.1007/BF00339607
Gordenin, D. A. ; Trofimova, M. V. ; Shaburova, O. N. ; Pavlov, Youri I ; Chernoff, Y. O. ; Chekuolene, Y. V. ; Proscyavichus, Y. Y. ; Sasnauskas, K. V. ; Janulaitis, A. A. / Precise excision of bacterial transposon Tn 5 in yeast. In: MGG Molecular & General Genetics. 1988 ; Vol. 213, No. 2-3. pp. 388-393.
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