Precise engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers

Frank Gu, Liangfang Zhang, Benjamin A. Teply, Nina Mann, Andrew Wang, Aleksandar F. Radovic-Moreno, Robert Langer, Omid C. Farokhzad

Research output: Contribution to journalArticle

519 Citations (Scopus)

Abstract

There has been progressively heightened interest in the development of targeted nanoparticles (NPs) for differential delivery and controlled release of drugs. Despite nearly three decades of research, approaches to reproducibly formulate targeted NPs with the optimal biophysicochemical properties have remained elusive. A central challenge has been defining the optimal interplay of parameters that confer molecular targeting, immune evasion, and drug release to overcome the physiological barriers in vivo. Here, wereport a strategy for narrowly changing the biophysicochemical properties of NPs in a reproducible manner, thereby enabling systematic screening of optimally formulated drug-encapsulated targeted NPs. NPs were formulated by the self-assembly of an amphiphilic triblock copolymer composed of end-to-end linkage of poly(lactic-co-glycolic-acid) (PLGA), polyethyleneglycol (PEG), and the A10 aptamer (Apt), which binds to the prostate-specific membrane antigen (PSMA) on the surface of prostate cancer (PCa) cells, enabling, respectively, controlled drug release, "stealth" properties for immune evasion, and cell-specific targeting. Fine-tuning of NP size and drug release kinetics was further accomplished by controlling the copolymer composition. By using distinct ratios of PLGA-b-PEG-b-Apt triblock copolymer with PLGA-b-PEG diblock copolymer lacking the A10 Apt, we developed a series of targeted NPs with increasing Apt densities that inversely affected the amount of PEG exposure on NP surface and identified the narrow range of Apt density when the NPs were maximally targeted and maximally stealth, resulting in most efficient PCa cell uptake in vitro and in vivo. This approach may contribute to further development of targeted NPs as highly selective and effective therapeutic modalities.

Original languageEnglish (US)
Pages (from-to)2586-2591
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number7
DOIs
StatePublished - Feb 19 2008

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Nanoparticles
Immune Evasion
antineoplaston A10
Prostatic Neoplasms
Pharmacokinetics
Drug Liberation
Research
Pharmaceutical Preparations

Keywords

  • Aptamer
  • Chemotherapy
  • Combinatorial screening
  • Controlled release

ASJC Scopus subject areas

  • General

Cite this

Precise engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers. / Gu, Frank; Zhang, Liangfang; Teply, Benjamin A.; Mann, Nina; Wang, Andrew; Radovic-Moreno, Aleksandar F.; Langer, Robert; Farokhzad, Omid C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 7, 19.02.2008, p. 2586-2591.

Research output: Contribution to journalArticle

Gu, Frank ; Zhang, Liangfang ; Teply, Benjamin A. ; Mann, Nina ; Wang, Andrew ; Radovic-Moreno, Aleksandar F. ; Langer, Robert ; Farokhzad, Omid C. / Precise engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 7. pp. 2586-2591.
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