Pre-osteoblastic MC3T3-E1 cells promote breast cancer growth in bone in a murine xenograft model

Thomas M. Bodenstine, Benjamin H. Beck, Xuemei Cao, Leah M. Cook, Aimen Ismail, J. Kent Powers, Andrea M. Mastro, Danny R. Welch

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The bones are the most common sites of breast cancer metastasis. Upon arrival within the bone microenvironment, breast cancer cells coordinate the activities of stromal cells, resulting in an increase in osteoclast activity and bone matrix degradation. In late stages of bone metastasis, breast cancer cells induce apoptosis in osteoblasts, which further exacerbates bone loss. However, in early stages, breast cancer cells induce osteoblasts to secrete inflammatory cytokines purported to drive tumor progression. To more thoroughly evaluate the role of osteoblasts in early stages of breast cancer metastasis to the bones, we used green fluorescent protein-la beled human breast cancer cell lines MDA-MB-23 1 and MDA-M B-43 5, which both induce osteolysis after intra-fe moral injection in athymic mice, and the murine pre-os teoblastic cell line MC3T3-E 1 to modulate osteoblast populations at the sites of breast cancer metastasis. Breast cancer cells were injected directly into the femur with or without equal numbers of MC3T3-E 1 cells. Tumors grew significantly larger when co-in jected with breast cancer cells and MC3T3-E 1 cells than injected with breast cancer cells alone. Osteolysis was induced in both groups, indicating that MC3T3-E 1 cells did not block the ability of breast cancer cells to cause bone destruction. MC3T3-E 1 cells promoted tumor growth out of the bone into the extraosseous stroma. These data suggest that breast cancer cells and osteoblasts communicate during early stages of bone metastasis and promote tumor growth.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalChinese Journal of Cancer
Volume30
Issue number3
DOIs
StatePublished - Jan 1 2011

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Bone Development
Heterografts
Breast Neoplasms
Osteoblasts
Bone and Bones
Neoplasm Metastasis
Osteolysis
Neoplasms
Cell Line
Bone Matrix
Osteoclasts
Stromal Cells
Green Fluorescent Proteins
Nude Mice
Femur
Apoptosis
Cytokines

Keywords

  • Bone
  • Breast cancer
  • MC3T3-E1
  • Metastasis
  • Osteoblast

ASJC Scopus subject areas

  • Oncology

Cite this

Bodenstine, T. M., Beck, B. H., Cao, X., Cook, L. M., Ismail, A., Kent Powers, J., ... Welch, D. R. (2011). Pre-osteoblastic MC3T3-E1 cells promote breast cancer growth in bone in a murine xenograft model. Chinese Journal of Cancer, 30(3), 189-196. https://doi.org/10.5732/cjc.010.10582

Pre-osteoblastic MC3T3-E1 cells promote breast cancer growth in bone in a murine xenograft model. / Bodenstine, Thomas M.; Beck, Benjamin H.; Cao, Xuemei; Cook, Leah M.; Ismail, Aimen; Kent Powers, J.; Mastro, Andrea M.; Welch, Danny R.

In: Chinese Journal of Cancer, Vol. 30, No. 3, 01.01.2011, p. 189-196.

Research output: Contribution to journalArticle

Bodenstine, TM, Beck, BH, Cao, X, Cook, LM, Ismail, A, Kent Powers, J, Mastro, AM & Welch, DR 2011, 'Pre-osteoblastic MC3T3-E1 cells promote breast cancer growth in bone in a murine xenograft model', Chinese Journal of Cancer, vol. 30, no. 3, pp. 189-196. https://doi.org/10.5732/cjc.010.10582
Bodenstine, Thomas M. ; Beck, Benjamin H. ; Cao, Xuemei ; Cook, Leah M. ; Ismail, Aimen ; Kent Powers, J. ; Mastro, Andrea M. ; Welch, Danny R. / Pre-osteoblastic MC3T3-E1 cells promote breast cancer growth in bone in a murine xenograft model. In: Chinese Journal of Cancer. 2011 ; Vol. 30, No. 3. pp. 189-196.
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