Potential use of Pseudomonas koreensis AGB-1 in association with Miscanthus sinensis to remediate heavy metal(loid)-contaminated mining site soil

A. Giridhar Babu, Patrick J. Shea, D. Sudhakar, Ik Boo Jung, Byung Taek Oh

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Endophytic bacteria have the potential to promote plant growth and heavy metal(loid) (HM) removal from contaminated soil. Pseudomonas koreensis AGB-1, isolated from roots of Miscanthus sinensis growing in mine-tailing soil, exhibited high tolerance to HMs and plant growth promoting traits. Transmission electron microscope (TEM) analysis revealed that AGB-1 sequestered HMs extracellularly and their accumulation was visible as dark metal complexes on bacterial surfaces and outside of the cells. DNA sequencing of HM resistance marker genes indicated high homology to the appropriate regions of the arsB, ACR3(1), aoxB, and bmtA determinants. Inoculating mining site soil with AGB-1 increased M.sinensis biomass by 54%, chlorophyll by 27%, and protein content by 28%. High superoxide dismutase and catalase activities, and the lower malondialdehyde content of plants growing in AGB-1-inoculated soil indicate reduced oxidative stress. Metal(loid) concentrations in roots and shoots of plants grown in inoculated soil were higher than those of the controls in pot trials with mine tailing soil. Results suggest that AGB-1 can be used in association with M.sinensis to promote phytostabilization and remediation of HM-contaminated sites.

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalJournal of Environmental Management
Volume151
DOIs
StatePublished - Mar 5 2015

Fingerprint

Heavy metals
Association reactions
heavy metal
Soils
soil
tailings
Tailings
metal
Oxidative stress
homology
Chlorophyll
Metal complexes
Remediation
chlorophyll
remediation
shoot
tolerance
Bacteria
Biomass
DNA

Keywords

  • Heavy metal(loid)s
  • Mine tailings
  • Miscanthus sinensis
  • Phytoremediation
  • Pseudomonas koreensis

ASJC Scopus subject areas

  • Environmental Engineering
  • Waste Management and Disposal
  • Management, Monitoring, Policy and Law

Cite this

Potential use of Pseudomonas koreensis AGB-1 in association with Miscanthus sinensis to remediate heavy metal(loid)-contaminated mining site soil. / Babu, A. Giridhar; Shea, Patrick J.; Sudhakar, D.; Jung, Ik Boo; Oh, Byung Taek.

In: Journal of Environmental Management, Vol. 151, 05.03.2015, p. 160-166.

Research output: Contribution to journalArticle

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abstract = "Endophytic bacteria have the potential to promote plant growth and heavy metal(loid) (HM) removal from contaminated soil. Pseudomonas koreensis AGB-1, isolated from roots of Miscanthus sinensis growing in mine-tailing soil, exhibited high tolerance to HMs and plant growth promoting traits. Transmission electron microscope (TEM) analysis revealed that AGB-1 sequestered HMs extracellularly and their accumulation was visible as dark metal complexes on bacterial surfaces and outside of the cells. DNA sequencing of HM resistance marker genes indicated high homology to the appropriate regions of the arsB, ACR3(1), aoxB, and bmtA determinants. Inoculating mining site soil with AGB-1 increased M.sinensis biomass by 54{\%}, chlorophyll by 27{\%}, and protein content by 28{\%}. High superoxide dismutase and catalase activities, and the lower malondialdehyde content of plants growing in AGB-1-inoculated soil indicate reduced oxidative stress. Metal(loid) concentrations in roots and shoots of plants grown in inoculated soil were higher than those of the controls in pot trials with mine tailing soil. Results suggest that AGB-1 can be used in association with M.sinensis to promote phytostabilization and remediation of HM-contaminated sites.",
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