Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil

Jaehong Shim, Patrick J Shea, Ik Boo Jung, Byung Taek Oh, Min Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The potential of a lead (Pb)-tolerant Pseudomonas sp. JH 51-2 strain to promote Pb stabilization was evaluated in mining site soil. The strain was isolated from soil containing multiple heavy metals. Transmission electron microscopy (TEM) showed that cell walls were surrounded by extracellular substances and scanning electron microscope-energy dispersive spectroscopy (SEM-EDS) confirmed the presence of Pb on the surface of cell membrane. Fourier transform infrared spectroscopy (FTIR) revealed that amino acids, phospholipids and extracellular polysaccharides were involved in Pb complexation and biosorption. Sequential extraction and toxicity characteristic leaching procedure (TCLP) showed decreased Pb availability in mining site soil in the presence of Pseudomonas sp. JH 51-2. Results indicate that Pseudomonas sp. JH 51-2 is an efficient biological stabilizer of Pb in contaminated soil.

Original languageEnglish (US)
Pages (from-to)e695-e701
JournalJournal of Environmental Biology
Volume36
Issue number3
StatePublished - May 1 2015

Fingerprint

Lead mines
Pseudomonas
Soil
Soils
Biosorption
Phospholipids
Fourier Transform Infrared Spectroscopy
Cell membranes
Polysaccharides
Heavy Metals
Complexation
Transmission Electron Microscopy
Cell Wall
Leaching
Heavy metals
Fourier transform infrared spectroscopy
Toxicity
Amino acids
Energy dispersive spectroscopy
Spectrum Analysis

Keywords

  • Biostabilization
  • Lead
  • Pseudomonas sp.
  • Sequential extraction
  • Toxicity characteristic leaching procedure

ASJC Scopus subject areas

  • Environmental Engineering
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil. / Shim, Jaehong; Shea, Patrick J; Jung, Ik Boo; Oh, Byung Taek; Cho, Min.

In: Journal of Environmental Biology, Vol. 36, No. 3, 01.05.2015, p. e695-e701.

Research output: Contribution to journalArticle

Shim, J, Shea, PJ, Jung, IB, Oh, BT & Cho, M 2015, 'Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil', Journal of Environmental Biology, vol. 36, no. 3, pp. e695-e701.
Shim, Jaehong ; Shea, Patrick J ; Jung, Ik Boo ; Oh, Byung Taek ; Cho, Min. / Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil. In: Journal of Environmental Biology. 2015 ; Vol. 36, No. 3. pp. e695-e701.
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