Potassium uptake modulates Staphylococcus aureus metabolism

Casey M. Gries, Marat R. Sadykov, Logan L. Bulock, Sujata S. Chaudhari, Vinai Chittezham Thomas, Jeffrey L. Bose, Kenneth W Bayles

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K+) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K+ uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K+ deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K+ uptake in maintaining essential components of S. aureus metabolism.

Original languageEnglish (US)
Article numbere00125-16
JournalmSphere
Volume1
Issue number3
DOIs
StatePublished - May 1 2016

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Staphylococcus aureus
Potassium
Homeostasis
Carbon Cycle
Proton-Motive Force
Oxidative Phosphorylation
Community Hospital
Cross Infection
Adenosine Triphosphate
Maintenance
Phosphorylation
Membranes

Keywords

  • Metabolism
  • Potassium transport
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Gries, C. M., Sadykov, M. R., Bulock, L. L., Chaudhari, S. S., Chittezham Thomas, V., Bose, J. L., & Bayles, K. W. (2016). Potassium uptake modulates Staphylococcus aureus metabolism. mSphere, 1(3), [e00125-16]. https://doi.org/10.1128/mSphere.00125-16

Potassium uptake modulates Staphylococcus aureus metabolism. / Gries, Casey M.; Sadykov, Marat R.; Bulock, Logan L.; Chaudhari, Sujata S.; Chittezham Thomas, Vinai; Bose, Jeffrey L.; Bayles, Kenneth W.

In: mSphere, Vol. 1, No. 3, e00125-16, 01.05.2016.

Research output: Contribution to journalArticle

Gries, CM, Sadykov, MR, Bulock, LL, Chaudhari, SS, Chittezham Thomas, V, Bose, JL & Bayles, KW 2016, 'Potassium uptake modulates Staphylococcus aureus metabolism', mSphere, vol. 1, no. 3, e00125-16. https://doi.org/10.1128/mSphere.00125-16
Gries CM, Sadykov MR, Bulock LL, Chaudhari SS, Chittezham Thomas V, Bose JL et al. Potassium uptake modulates Staphylococcus aureus metabolism. mSphere. 2016 May 1;1(3). e00125-16. https://doi.org/10.1128/mSphere.00125-16
Gries, Casey M. ; Sadykov, Marat R. ; Bulock, Logan L. ; Chaudhari, Sujata S. ; Chittezham Thomas, Vinai ; Bose, Jeffrey L. ; Bayles, Kenneth W. / Potassium uptake modulates Staphylococcus aureus metabolism. In: mSphere. 2016 ; Vol. 1, No. 3.
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