Potassium ion channels of chlorella viruses cause rapid depolarization of host cells during infection

Florian Frohns, Anja Käsmann, Detlef Kramer, Britta Schäfer, Mario Mehmel, Ming Kang, James L Van Etten, Sabrina Gazzarrini, Anna Moroni, Gerhard Thiel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Previous studies have established that chlorella viruses encode K + channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these channels to Cs+ to determine if the membrane depolarization observed during virus infection is caused by the activities of these channels. Infection of Chlorella NC64A with four viruses caused rapid membrane depolarization of similar amplitudes, but with different kinetics. Depolarization was fastest after infection with virus SC-1A (half time [t1/2], about 9 min) and slowest with virus NY-2A (t1/2, about 12 min). Cs+ inhibited membrane depolarization only in viruses that encode a Cs +-sensitive K+ channel. Collectively, the results indicate that membrane depolarization is an early event in chlorella virus-host interactions and that it is correlated with viral-channel activity. This suggestion was supported by investigations of thin sections of Chlorella cells, which show that channel blockers inhibit virus DNA release into the host cell. Together, the data indicate that the channel is probably packaged in the virion, presumably in its internal membrane. We hypothesize that fusion of the virus internal membrane with the host plasma membrane results in an increase in K + conductance and membrane depolarization; this depolarization lowers the energy barrier for DNA release into the host.

Original languageEnglish (US)
Pages (from-to)2437-2444
Number of pages8
JournalJournal of virology
Volume80
Issue number5
DOIs
StatePublished - Mar 1 2006

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Chlorella
Potassium Channels
ion channels
potassium
Viruses
viruses
Membranes
Infection
infection
Virus Diseases
cells
potassium channels
Virus Release
Virus Internalization
Virion
DNA viruses
virion
Cell Membrane
functional properties
plasma membrane

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Frohns, F., Käsmann, A., Kramer, D., Schäfer, B., Mehmel, M., Kang, M., ... Thiel, G. (2006). Potassium ion channels of chlorella viruses cause rapid depolarization of host cells during infection. Journal of virology, 80(5), 2437-2444. https://doi.org/10.1128/JVI.80.5.2437-2444.2006

Potassium ion channels of chlorella viruses cause rapid depolarization of host cells during infection. / Frohns, Florian; Käsmann, Anja; Kramer, Detlef; Schäfer, Britta; Mehmel, Mario; Kang, Ming; Van Etten, James L; Gazzarrini, Sabrina; Moroni, Anna; Thiel, Gerhard.

In: Journal of virology, Vol. 80, No. 5, 01.03.2006, p. 2437-2444.

Research output: Contribution to journalArticle

Frohns, F, Käsmann, A, Kramer, D, Schäfer, B, Mehmel, M, Kang, M, Van Etten, JL, Gazzarrini, S, Moroni, A & Thiel, G 2006, 'Potassium ion channels of chlorella viruses cause rapid depolarization of host cells during infection', Journal of virology, vol. 80, no. 5, pp. 2437-2444. https://doi.org/10.1128/JVI.80.5.2437-2444.2006
Frohns, Florian ; Käsmann, Anja ; Kramer, Detlef ; Schäfer, Britta ; Mehmel, Mario ; Kang, Ming ; Van Etten, James L ; Gazzarrini, Sabrina ; Moroni, Anna ; Thiel, Gerhard. / Potassium ion channels of chlorella viruses cause rapid depolarization of host cells during infection. In: Journal of virology. 2006 ; Vol. 80, No. 5. pp. 2437-2444.
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