Posttranscriptional Regulation of the c-myb Proto-oncogene in Estrogen Receptor-Positive Breast Cancer Cells

Jean M. Gudas, Regina C. Klein, Kenneth H Cowan, Mikio Oka

Research output: Contribution to journalArticle

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Abstract

We have determined that expression of the c-myb protooncogene is associated with estrogen receptor (ER) status and not with tumor progression in human breast epithelial cells. Analysis of normal, immortalized, nontumorigenic, and tumorigenic mammary epithelial cells showed that only ER+ tumor cell lines expressed readily detectable levels of c-myb mRNA and a Mr 75,000 protein that was the same size as the c-myb transcripts and protein products present in hematopoietic cells. In this report we show that c-myb mRNA and protein levels are down-regulated during estrogen withdrawal. A 20-fold increase in c-myb mRNA and protein expression was observed upon addition of β-estradiol to the culture medium. Nuclear run-on transcription analyses showed that c-myb was transcribed at the same rate in the presence and absence of estrogen, suggesting that c-myb mRNA accumulation was regulated at a posttranscriptional level. To provide additional evidence that c-myb mRNA was dependent on ER expression, we examined c-myb mRNA levels in MCF-7 cells selected for resistance to antineoplastic drugs, c-myb expression was decreased only in cell lines that showed concomitant loss of ER expression. Moreover, c-myb mRNA was expressed and modulated by estrogen in ER~, MDA-MB-231 cells stably transfected with a human ER gene. When considered together, these data indicate that c-myb mRNA levels are regulated by estrogens and further suggest that this proto-oncogene plays a role in the biology of ER+ breast tumor cells.

Original languageEnglish (US)
Pages (from-to)235-243
Number of pages9
JournalClinical Cancer Research
Volume1
Issue number2
StatePublished - Feb 1 1995

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myb Genes
Estrogen Receptors
Breast Neoplasms
Proto-Oncogene Proteins c-myb
Messenger RNA
Estrogens
Neoplasm Drug Resistance
Breast
Epithelial Cells
Proto-Oncogenes
MCF-7 Cells
Tumor Cell Line
Culture Media
Estradiol
Cell Line

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Posttranscriptional Regulation of the c-myb Proto-oncogene in Estrogen Receptor-Positive Breast Cancer Cells. / Gudas, Jean M.; Klein, Regina C.; Cowan, Kenneth H; Oka, Mikio.

In: Clinical Cancer Research, Vol. 1, No. 2, 01.02.1995, p. 235-243.

Research output: Contribution to journalArticle

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