Possible existence of PepT1 in pigmented rabbit conjunctiva

S. K. Basu, L. Sun, F. F. Lin, Ming-Fong Lin, D. Ann, K. J. Kim, V. H L Lee

Research output: Contribution to journalArticle

Abstract

Purpose: To investigate the existence of dipeptide transporter PepT1 at the mRNA level in the pigmented rabbit conjunctiva. Methods: Total RNA was isolated from the conjunctival and the jejunal (positive control) epithelial cells using the guanidine hvdrochloride-chloroform-isopropanol method. The amount of total RNA was determined and the integrity of RNA was analyzed. Complementary DNA was made by using total RNA and reverse transcriptase, which was then amplified using polymerase chain reaction. Two primers (bases 31 to 50 and 1031 to 1050) were synthesized based on the rabbit jejunal oligopeptide transporter PepT1 cDNA sequence. The length of PCR product was predicted to be 1.0 kb. The RT-PCR products were analyzed with 1% agarose gel and stained with ethidium bromide. Results: RT-PCR products revealed a positive band at 1.0 kb for both the conjunctival and the jejunal epithelial samples. The signal intensity for the band from the conjunctival epithelial sample, however, was lesser than that from the jejunal sample when equal amounts of RNA were used. This suggests a lower level of expression for PepT1 in the conjunctiva compared to the jejunum. Conclusions: Previously we have demonstrated H+-coupled dipeptide transport properties in the pigmented rabbit conjunctiva. Although the expression level at the mRNA level is lower in the conjunctiva compared to the jejunum, these results coupled with our earlier findings suggest the possibe existence of dipeptide transporter in the pigmented rabbit conjunctiva.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

Fingerprint

Conjunctiva
Dipeptides
Rabbits
RNA
Polymerase Chain Reaction
RNA-Directed DNA Polymerase
Jejunum
Complementary DNA
Oligopeptides
Messenger RNA
Ethidium
2-Propanol
Guanidine
Chloroform
Sepharose
Gels
Epithelial Cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Basu, S. K., Sun, L., Lin, F. F., Lin, M-F., Ann, D., Kim, K. J., & Lee, V. H. L. (1996). Possible existence of PepT1 in pigmented rabbit conjunctiva. Investigative Ophthalmology and Visual Science, 37(3).

Possible existence of PepT1 in pigmented rabbit conjunctiva. / Basu, S. K.; Sun, L.; Lin, F. F.; Lin, Ming-Fong; Ann, D.; Kim, K. J.; Lee, V. H L.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Basu, SK, Sun, L, Lin, FF, Lin, M-F, Ann, D, Kim, KJ & Lee, VHL 1996, 'Possible existence of PepT1 in pigmented rabbit conjunctiva', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Basu, S. K. ; Sun, L. ; Lin, F. F. ; Lin, Ming-Fong ; Ann, D. ; Kim, K. J. ; Lee, V. H L. / Possible existence of PepT1 in pigmented rabbit conjunctiva. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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