Porcine kallikrein gene family

Genomic structure, mapping, and differential expression analysis

Samodha C Fernando, F. Z. Najar, X. Guo, L. Zhou, Y. Fu, R. D. Geisert, B. A. Roe, U. DeSilva

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Kallikreins belong to a family of serine proteases that are widespread throughout living organisms, expressed in diverse tissue-specific patterns, and known to have highly diverse physiological functions. The 15 human and 24 mouse kallikreins have been implicated in pathophysiology of brain, kidney, and respiratory and reproductive systems and often are used as cancer biomarkers. To better elucidate the structure and evolutionary origin of this important gene family in the pig, we have constructed a contiguous BAC clone-derived physical map of the porcine kallikrein gene region and have fully sequenced a BAC clone containing 13 kallikrein genes, 11 of which are novel. Radiation hybrid mapping assigns this kallikrein-gene-rich region to porcine chromosome 6. Phylogenetic and percent identity plot-based analyses revealed strong structure and order conservation of kallikreins among four mammalian species. Reverse transcriptase-polymerase chain reaction-based expression analysis of porcine kallikreins showed a complex expression pattern across different tissues with the thymus being the only tissue expressing all 13 kallikrein genes. [The sequence data described in this paper has been submitted to GenBank under Accession No. AC149292].

Original languageEnglish (US)
Pages (from-to)429-438
Number of pages10
JournalGenomics
Volume89
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Kallikreins
Swine
Genes
Clone Cells
Radiation Hybrid Mapping
Chromosomes, Human, Pair 6
Nucleic Acid Databases
Serine Proteases
Tumor Biomarkers
Reverse Transcriptase Polymerase Chain Reaction
Respiratory System
Thymus Gland
Kidney
Brain

Keywords

  • Comparative genomics
  • Kallikrein
  • Physical mapping
  • Pig
  • Porcine
  • RH mapping
  • Radiation hybrid mapping
  • Serine proteases

ASJC Scopus subject areas

  • Genetics

Cite this

Porcine kallikrein gene family : Genomic structure, mapping, and differential expression analysis. / Fernando, Samodha C; Najar, F. Z.; Guo, X.; Zhou, L.; Fu, Y.; Geisert, R. D.; Roe, B. A.; DeSilva, U.

In: Genomics, Vol. 89, No. 3, 01.03.2007, p. 429-438.

Research output: Contribution to journalArticle

Fernando, SC, Najar, FZ, Guo, X, Zhou, L, Fu, Y, Geisert, RD, Roe, BA & DeSilva, U 2007, 'Porcine kallikrein gene family: Genomic structure, mapping, and differential expression analysis', Genomics, vol. 89, no. 3, pp. 429-438. https://doi.org/10.1016/j.ygeno.2006.11.010
Fernando, Samodha C ; Najar, F. Z. ; Guo, X. ; Zhou, L. ; Fu, Y. ; Geisert, R. D. ; Roe, B. A. ; DeSilva, U. / Porcine kallikrein gene family : Genomic structure, mapping, and differential expression analysis. In: Genomics. 2007 ; Vol. 89, No. 3. pp. 429-438.
@article{c0237fae3f7c4c7082ce5dc3af682926,
title = "Porcine kallikrein gene family: Genomic structure, mapping, and differential expression analysis",
abstract = "Kallikreins belong to a family of serine proteases that are widespread throughout living organisms, expressed in diverse tissue-specific patterns, and known to have highly diverse physiological functions. The 15 human and 24 mouse kallikreins have been implicated in pathophysiology of brain, kidney, and respiratory and reproductive systems and often are used as cancer biomarkers. To better elucidate the structure and evolutionary origin of this important gene family in the pig, we have constructed a contiguous BAC clone-derived physical map of the porcine kallikrein gene region and have fully sequenced a BAC clone containing 13 kallikrein genes, 11 of which are novel. Radiation hybrid mapping assigns this kallikrein-gene-rich region to porcine chromosome 6. Phylogenetic and percent identity plot-based analyses revealed strong structure and order conservation of kallikreins among four mammalian species. Reverse transcriptase-polymerase chain reaction-based expression analysis of porcine kallikreins showed a complex expression pattern across different tissues with the thymus being the only tissue expressing all 13 kallikrein genes. [The sequence data described in this paper has been submitted to GenBank under Accession No. AC149292].",
keywords = "Comparative genomics, Kallikrein, Physical mapping, Pig, Porcine, RH mapping, Radiation hybrid mapping, Serine proteases",
author = "Fernando, {Samodha C} and Najar, {F. Z.} and X. Guo and L. Zhou and Y. Fu and Geisert, {R. D.} and Roe, {B. A.} and U. DeSilva",
year = "2007",
month = "3",
day = "1",
doi = "10.1016/j.ygeno.2006.11.010",
language = "English (US)",
volume = "89",
pages = "429--438",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Porcine kallikrein gene family

T2 - Genomic structure, mapping, and differential expression analysis

AU - Fernando, Samodha C

AU - Najar, F. Z.

AU - Guo, X.

AU - Zhou, L.

AU - Fu, Y.

AU - Geisert, R. D.

AU - Roe, B. A.

AU - DeSilva, U.

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Kallikreins belong to a family of serine proteases that are widespread throughout living organisms, expressed in diverse tissue-specific patterns, and known to have highly diverse physiological functions. The 15 human and 24 mouse kallikreins have been implicated in pathophysiology of brain, kidney, and respiratory and reproductive systems and often are used as cancer biomarkers. To better elucidate the structure and evolutionary origin of this important gene family in the pig, we have constructed a contiguous BAC clone-derived physical map of the porcine kallikrein gene region and have fully sequenced a BAC clone containing 13 kallikrein genes, 11 of which are novel. Radiation hybrid mapping assigns this kallikrein-gene-rich region to porcine chromosome 6. Phylogenetic and percent identity plot-based analyses revealed strong structure and order conservation of kallikreins among four mammalian species. Reverse transcriptase-polymerase chain reaction-based expression analysis of porcine kallikreins showed a complex expression pattern across different tissues with the thymus being the only tissue expressing all 13 kallikrein genes. [The sequence data described in this paper has been submitted to GenBank under Accession No. AC149292].

AB - Kallikreins belong to a family of serine proteases that are widespread throughout living organisms, expressed in diverse tissue-specific patterns, and known to have highly diverse physiological functions. The 15 human and 24 mouse kallikreins have been implicated in pathophysiology of brain, kidney, and respiratory and reproductive systems and often are used as cancer biomarkers. To better elucidate the structure and evolutionary origin of this important gene family in the pig, we have constructed a contiguous BAC clone-derived physical map of the porcine kallikrein gene region and have fully sequenced a BAC clone containing 13 kallikrein genes, 11 of which are novel. Radiation hybrid mapping assigns this kallikrein-gene-rich region to porcine chromosome 6. Phylogenetic and percent identity plot-based analyses revealed strong structure and order conservation of kallikreins among four mammalian species. Reverse transcriptase-polymerase chain reaction-based expression analysis of porcine kallikreins showed a complex expression pattern across different tissues with the thymus being the only tissue expressing all 13 kallikrein genes. [The sequence data described in this paper has been submitted to GenBank under Accession No. AC149292].

KW - Comparative genomics

KW - Kallikrein

KW - Physical mapping

KW - Pig

KW - Porcine

KW - RH mapping

KW - Radiation hybrid mapping

KW - Serine proteases

UR - http://www.scopus.com/inward/record.url?scp=33846799014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846799014&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2006.11.010

DO - 10.1016/j.ygeno.2006.11.010

M3 - Article

VL - 89

SP - 429

EP - 438

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -