Poorly-controlled type 1 diabetes mellitus impairs LH-LHCGR signaling in the ovaries and decreases female fertility in mice

Jaewang Lee, Hoi Chang Lee, So Youn Kim, Geum Joon Cho, Teresa K. Woodruff

Research output: Contribution to journalArticle

Abstract

Purpose: The aim of this study was to investigate how type I diabetes mellitus (T1D) affects the folliculogenesis and oocyte development, fertilization, and embryo development. Materials and Methods: A comparative animal study was conducted using two different mouse models of T1D, a genetic AKITA model and a streptozotocin-induced diabetes model. Ovarian function was assessed by gross observation, immunoblot, immunohistochemistry, oocyte counting, and ELISA for serum hormones (insulin, anti-Mullerian hormone, estradiol, testosterone, and progesterone). Maturation and developmental competence of metaphase II oocytes from control and T1D animals was evaluated by immunofluorescent and immunohistochemical detection of biomarkers and in vitro fertilization. Results: Animals from both T1D models showed increased blood glucose levels, while only streptozotocin (STZ)-injected mice showed reduced body weight. Folliculogenesis, oogenesis, and preimplantation embryogenesis were impaired in both T1D mouse models. Interestingly, exogenous streptozotocin injection to induce T1D led to marked decreases in ovary size, expression of luteinizing hormone/chorionic gonadotropin receptor in the ovaries, the number of corpora lutea per ovary, oocyte maturation, and serum progesterone levels. Both T1D models exhibited significantly reduced pre-implantation embryo quality compared with controls. There was no significant difference in embryo quality between STZ-injected and AKITA diabetic mice. Conclusion: These results suggest that T1D affects folliculogenesis, oogenesis, and embryo development in mice. However, the physiological mechanisms underlying the observed reproductive effects of diabetes need to be further investigated.

Original languageEnglish (US)
Pages (from-to)667-678
Number of pages12
JournalYonsei Medical Journal
Volume60
Issue number7
DOIs
StatePublished - Jul 2019

Fingerprint

Type 1 Diabetes Mellitus
Fertility
Ovary
Oocytes
Streptozocin
Embryonic Development
Oogenesis
Progesterone
Anti-Mullerian Hormone
LH Receptors
Experimental Diabetes Mellitus
Corpus Luteum
Genetic Models
Fertilization in Vitro
Metaphase
Luteinizing Hormone
Serum
Fertilization
Mental Competency
Blood Glucose

Keywords

  • Folliculogenesis
  • Oocyte
  • Reproduction
  • Signalling
  • Type 1 diabetes mellitus

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Poorly-controlled type 1 diabetes mellitus impairs LH-LHCGR signaling in the ovaries and decreases female fertility in mice. / Lee, Jaewang; Lee, Hoi Chang; Kim, So Youn; Cho, Geum Joon; Woodruff, Teresa K.

In: Yonsei Medical Journal, Vol. 60, No. 7, 07.2019, p. 667-678.

Research output: Contribution to journalArticle

Lee, Jaewang ; Lee, Hoi Chang ; Kim, So Youn ; Cho, Geum Joon ; Woodruff, Teresa K. / Poorly-controlled type 1 diabetes mellitus impairs LH-LHCGR signaling in the ovaries and decreases female fertility in mice. In: Yonsei Medical Journal. 2019 ; Vol. 60, No. 7. pp. 667-678.
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