Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase

Kevser Biberoglu, Lawrence M Schopfer, Ashima Saxena, Ozden Tacal, Oksana Lockridge

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Acetylcholinesterase (AChE) in the serum of fetal cow is a tetramer. The related enzyme, butyrylcholinesterase (BChE), in the sera of humans and horse requires polyproline peptides for assembly into tetramers. Our goal was to determine whether soluble tetrameric AChE includes tetramer organizing peptides in its structure. Fetal bovine serum AChE was denatured by boiling to release non-covalently bound peptides. Bulk protein was separated from peptides by filtration and by high performance liquid chromatography. Peptide mass and amino acid sequence of the released peptides were determined by MALDI-TOF-TOF and LTQ-Orbitrap mass spectrometry. Twenty polyproline peptides, divided into 5 families, were identified. The longest peptide contained 25 consecutive prolines and no other amino acid. Other polyproline peptides included one non-proline amino acid, for example serine at the C-terminus of 20 prolines. A search of the mammalian proteome database suggested that this assortment of polyproline peptides originated from at least 5 different precursor proteins, none of which were the ColQ or PRiMA of membrane-anchored AChE. To date, AChE and BChE are the only proteins known that include polyproline tetramer organizing peptides in their tetrameric structure.

Original languageEnglish (US)
Pages (from-to)745-753
Number of pages9
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1834
Issue number4
DOIs
StatePublished - Apr 1 2013

Fingerprint

Acetylcholinesterase
Peptides
Serum
Butyrylcholinesterase
Amino Acids
Proline
polyproline
Protein Precursors
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
High performance liquid chromatography
Proteome
Boiling liquids
Serine
Horses
Mass spectrometry
Amino Acid Sequence
Mass Spectrometry
Proteins
High Pressure Liquid Chromatography
Databases

Keywords

  • Fetal bovine serum acetylcholinesterase
  • Mass spectrometry
  • PAGE gel electrophoresis
  • Polyproline peptide
  • Tetramer organization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Polyproline tetramer organizing peptides in fetal bovine serum acetylcholinesterase. / Biberoglu, Kevser; Schopfer, Lawrence M; Saxena, Ashima; Tacal, Ozden; Lockridge, Oksana.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1834, No. 4, 01.04.2013, p. 745-753.

Research output: Contribution to journalArticle

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