Polypeptide Core of a Human Pancreatic Tumor Mucin Antigen

Michael S. Lan, Michael A. Hollingsworth, Richard S. Metzgar

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

This work describes the molecular properties of the polypeptide core of a human pancreatic mucin antigen isolated from a human pancreatic adenocarcinoma cell line, HPAF. Pancreatic tumor mucin was isolated by a combination of molecular sieve chromatography and CsCl/4 m guanidine-HCl density gradient ultracentrifugation. Trifluoromethane sulfonic acid was used to remove carbohydrate units from purified mucin molecules. A rabbit monospecific polyclonal antibody was generated against pancreatic apomucin and reacted with a Mt < 200,000 species. The antibody binding data indicated that the rabbit antiserum raised against pancreatic apomucin cross-reacted with a breast mucin synthetic peptide. Northern blot and immunodot blot analyses of various cell line extracts revealed that a tandem repeat sequence and a similar mRNA were detected in both pancreatic and breast mucin-producing cell lines. These results suggest that pancreatic apomucin and breast apomucin share some similarity in tandem repeated nucleic acid and protein sequences.

Original languageEnglish (US)
Pages (from-to)2997-3001
Number of pages5
JournalCancer Research
Volume50
Issue number10
StatePublished - May 15 1990

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Neoplasm Antigens
Mucins
Peptides
Breast
Cell Line
Rabbits
Tandem Repeat Sequences
Sulfonic Acids
Antibodies
Ultracentrifugation
Guanidine
Cell Extracts
Northern Blotting
Nucleic Acids
Gel Chromatography
Immune Sera
Adenocarcinoma
Carbohydrates
Antigens
Messenger RNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Polypeptide Core of a Human Pancreatic Tumor Mucin Antigen. / Lan, Michael S.; Hollingsworth, Michael A.; Metzgar, Richard S.

In: Cancer Research, Vol. 50, No. 10, 15.05.1990, p. 2997-3001.

Research output: Contribution to journalArticle

Lan, MS, Hollingsworth, MA & Metzgar, RS 1990, 'Polypeptide Core of a Human Pancreatic Tumor Mucin Antigen', Cancer Research, vol. 50, no. 10, pp. 2997-3001.
Lan, Michael S. ; Hollingsworth, Michael A. ; Metzgar, Richard S. / Polypeptide Core of a Human Pancreatic Tumor Mucin Antigen. In: Cancer Research. 1990 ; Vol. 50, No. 10. pp. 2997-3001.
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