Polyoxazoline‐Peptide adducts that retain antibody avidity

William H. Velander, Rapti D. Madurawe, Anuradha Subramanian, Guneet Kumar, Gurudas Sinai‐Zingde, Judy S. Riffle, Carolyn L. Orthner

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43 Scopus citations

Abstract

Synthetic polymers have long been used to modify various properties of proteins such as activity and solubility. Polyethylene glycol (PEG) has been widely used to form adducts with enzymes and antibodies. In this study, the polyoxazoline family of water‐soluble polymers was used to synthesize adducts containing a synthetic peptide recognized by a monoclonal antibody (MAb) directed against human protein C (hPC). This is the first application of direct conjugation of unterminated or “living” polymer to a peptide. The avidity of the antibody for the various adducts was characterized with respect to size and hydrophilicity of methyl‐ and ethyl‐substituted polyoxazoline polymers (POX). Avidity of the adducts was not found to be dependent upon the hydrophilicity and was slightly decreased due to polymer modification. The methyl‐POX‐peptide adducts were found to be highly water soluble, while the ethyl‐POX‐peptide adducts showed sporadic problems with aqueous solubility. Because the polymer‐peptide adducts retained avidity for the antibody, polyoxazoline polymers may have potential application to protein‐adduct chemistry.

Original languageEnglish (US)
Pages (from-to)1024-1030
Number of pages7
JournalBiotechnology and Bioengineering
Volume39
Issue number10
DOIs
StatePublished - Apr 25 1992

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Keywords

  • monoclonal antibodies
  • polymer‐peptide adducts
  • polyoxazoline

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Velander, W. H., Madurawe, R. D., Subramanian, A., Kumar, G., Sinai‐Zingde, G., Riffle, J. S., & Orthner, C. L. (1992). Polyoxazoline‐Peptide adducts that retain antibody avidity. Biotechnology and Bioengineering, 39(10), 1024-1030. https://doi.org/10.1002/bit.260391006