Polymeric micelleplexes for improved photothermal endosomal escape and delivery of siRNA

Gang Chen, Ling Ding, Pengkai Wu, Yiwen Zhou, Minjie Sun, Kaikai Wang, David Oupický

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Effective endosomal escape is required for practical application of nucleic acid therapeutics. In this study, we prepared siRNA micelleplexes with photothermally triggered endosomal escape and improved gene silencing activity in vitro. The micelleplexes were prepared from cholesterol-modified and CXCR4-inhibiting poly(amido amine)s (PAMD-Ch). Near-infrared dye IR780 was encapsulated in cationic PMAD-Ch micelles, which then were used to form IR780 micelleplexes with siRNA. The micelleplexes displayed improved gene silencing efficiency upon laser irradiation, which was attributed to enhanced endosomal escape because of the photothermal effects of the encapsulated IR780. The IR780 micelleplexes retained the CXCR4 antagonism and inhibition of cancer cell invasion of the parent PAMD. Overall, this study validates codelivery of IR780 in siRNA micelleplexes as promising photothermally controlled siRNA delivery approach.

Original languageEnglish (US)
Pages (from-to)2593-2600
Number of pages8
JournalPolymers for Advanced Technologies
Volume29
Issue number10
DOIs
StatePublished - Oct 2018

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Keywords

  • IR780
  • endosomal escape
  • photothermal
  • polyplex
  • siRNA delivery

ASJC Scopus subject areas

  • Polymers and Plastics

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