Polyclonal antibody to soman-tyrosine

Bin Li, Ellen G. Duysen, Marie Thérèse Froment, Patrick Masson, Florian Nachon, Wei Jiang, Lawrence M Schopfer, Geoffrey Milton Thiele, Lynell Warren Klassen, John Cashman, Gareth R. Williams, Oksana Lockridge

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Soman forms a stable, covalent bond with tyrosine 411 of human albumin, with tyrosines 257 and 593 in human transferrin, and with tyrosine in many other proteins. The pinacolyl group of soman is retained, suggesting that pinacolyl methylphosphonate bound to tyrosine could generate specific antibodies. Tyrosine in the pentapeptide RYGRK was covalently modified with soman simply by adding soman to the peptide. The phosphonylated-peptide was linked to keyhole limpet hemocyanin, and the conjugate was injected into rabbits. The polyclonal antiserum recognized soman-labeled human albumin, soman-mouse albumin, and soman human transferrin but not nonphosphonylated control proteins. The soman-labeled tyrosines in these proteins are surrounded by different amino acid sequences, suggesting that the polyclonal recognizes soman-tyrosine independent of the amino acid sequence. Antiserum obtained after 4 antigen injections over a period of 18 weeks was tested in a competition ELISA where it had an IC50 of 10-11 M. The limit of detection on Western blots was 0.01 μg (15 picomoles) of soman-labeled albumin. In conclusion, a high-affinity, polyclonal antibody that specifically recognizes soman adducts on tyrosine in a variety of proteins has been produced. Such an antibody could be useful for identifying secondary targets of soman toxicity.

Original languageEnglish (US)
Pages (from-to)584-592
Number of pages9
JournalChemical Research in Toxicology
Volume26
Issue number4
DOIs
StatePublished - Apr 15 2013

Fingerprint

Soman
Tyrosine
Antibodies
Albumins
Transferrin
Immune Sera
Amino Acid Sequence
Proteins
Amino Acids
Peptides
Antibody Affinity
Covalent bonds
Inhibitory Concentration 50
Toxicity
Limit of Detection

ASJC Scopus subject areas

  • Toxicology

Cite this

Li, B., Duysen, E. G., Froment, M. T., Masson, P., Nachon, F., Jiang, W., ... Lockridge, O. (2013). Polyclonal antibody to soman-tyrosine. Chemical Research in Toxicology, 26(4), 584-592. https://doi.org/10.1021/tx400027n

Polyclonal antibody to soman-tyrosine. / Li, Bin; Duysen, Ellen G.; Froment, Marie Thérèse; Masson, Patrick; Nachon, Florian; Jiang, Wei; Schopfer, Lawrence M; Thiele, Geoffrey Milton; Klassen, Lynell Warren; Cashman, John; Williams, Gareth R.; Lockridge, Oksana.

In: Chemical Research in Toxicology, Vol. 26, No. 4, 15.04.2013, p. 584-592.

Research output: Contribution to journalArticle

Li, B, Duysen, EG, Froment, MT, Masson, P, Nachon, F, Jiang, W, Schopfer, LM, Thiele, GM, Klassen, LW, Cashman, J, Williams, GR & Lockridge, O 2013, 'Polyclonal antibody to soman-tyrosine', Chemical Research in Toxicology, vol. 26, no. 4, pp. 584-592. https://doi.org/10.1021/tx400027n
Li B, Duysen EG, Froment MT, Masson P, Nachon F, Jiang W et al. Polyclonal antibody to soman-tyrosine. Chemical Research in Toxicology. 2013 Apr 15;26(4):584-592. https://doi.org/10.1021/tx400027n
Li, Bin ; Duysen, Ellen G. ; Froment, Marie Thérèse ; Masson, Patrick ; Nachon, Florian ; Jiang, Wei ; Schopfer, Lawrence M ; Thiele, Geoffrey Milton ; Klassen, Lynell Warren ; Cashman, John ; Williams, Gareth R. ; Lockridge, Oksana. / Polyclonal antibody to soman-tyrosine. In: Chemical Research in Toxicology. 2013 ; Vol. 26, No. 4. pp. 584-592.
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AU - Jiang, Wei

AU - Schopfer, Lawrence M

AU - Thiele, Geoffrey Milton

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