Polyamine and macromolecule levels during spore germination in Rhizopus stolonifer and Botryodiplodia theobromae

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Abstract

Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalExperimental Mycology
Volume1
Issue number4
DOIs
StatePublished - Dec 1977

Fingerprint

Rhizopus
Polyamines
Germination
Spores
Putrescine
Spermidine
Fungi
RNA
DNA
Proteins

Keywords

  • Botryodiplodia theobromae
  • DNA
  • RNA
  • polyamines
  • protein
  • spores, Rhizopus stolonifer

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

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title = "Polyamine and macromolecule levels during spore germination in Rhizopus stolonifer and Botryodiplodia theobromae",
abstract = "Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.",
keywords = "Botryodiplodia theobromae, DNA, RNA, polyamines, protein, spores, Rhizopus stolonifer",
author = "Nickerson, {Kenneth W.} and McCune, {Bryan K.} and {Van Etten}, {James L.}",
year = "1977",
month = "12",
doi = "10.1016/S0147-5975(77)80007-8",
language = "English (US)",
volume = "1",
pages = "317--322",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "4",

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TY - JOUR

T1 - Polyamine and macromolecule levels during spore germination in Rhizopus stolonifer and Botryodiplodia theobromae

AU - Nickerson, Kenneth W.

AU - McCune, Bryan K.

AU - Van Etten, James L.

PY - 1977/12

Y1 - 1977/12

N2 - Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.

AB - Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.

KW - Botryodiplodia theobromae

KW - DNA

KW - RNA

KW - polyamines

KW - protein

KW - spores, Rhizopus stolonifer

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