Polyadenylate-containing RNA in dormant and germinating sporangiospores of Rhizopus stolonifer

James L. Van Etten, Shelby N. Freer

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Polyadenylic acid [poly(A)]segments and polyadenylate-containing [poly(A+)] RNAs were isolated from dormant and germinating sporangiospores of Rhizopus stolonifer. At all stages of germination the poly(A) segments (i) composed about 0.1% of the total RNA, (ii) averaged about 33 residues in length, and (iii) were attached to large heterodisperse RNA molecules. From this information and knowing the weight of RNA per spore, we estimated that each dormant spore contained about 6.6 × 105 molecules of poly(A+) RNA. Translation of the spore poly(A+) RNA in vitro revealed that at least some of these molecules were functional messenger RNAs and that they coded for the synthesis of many discrete polypeptides.

Original languageEnglish (US)
Pages (from-to)301-312
Number of pages12
JournalExperimental Mycology
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 1978

    Fingerprint

Keywords

  • RNA
  • Rhizopus stolonifer
  • messenger
  • poly(A)
  • spores
  • translation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this