Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage

Xuesong Liu, Xu Luo, Yan Shi, Gui Dong Zhu, Thomas Penning, Vincent L. Giranda, Yan Luo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Majority of chemotherapeutic agents inhibit tumor growth by inducing apoptosis or necrosis. The DNA alkylating agent, N-methyl-N′-nitro-N- nitrosoguanidine (MNNG), kills cells by necrosis through massive production of DNA strand breaks and subsequent over-activation of PARP. Inhibition of PARP, either through PARP1 genetic ablation or through small molecule PARP inhibitors, protected MNNG-induced cell death in certain cell types including MEF and primary cortical cultures. We report here that a potent PARP inhibitor, ABT-888, facilitates the induction of apoptotic cell death in HeLa cells treated with MNNG. Although the release of cytochrome c from mitochondria to cytosol was observed in HeLa cells treated with either MNNG alone or the combination of MNNG and ABT-888 (MNNG/ABT-888), apoptosis is observed only in HeLa cells treated with MNNG/ ABT-888. Bcl-2 family proteins regulate the release of cytochrome c. Downregulation of Bax and Bak by their corresponding siRNAs or overexpression of Bcl-xl inhibited the release of cytochrome c from mitochondria to cytosol, and inhibited apoptosis induced by MNNG/ABT-888. Further examination indicates that ATP concentration is greatly reduced in HeLa cells treated with MNNG alone, but not in HeLa cells treated with MNNG/ABT-888. Reduction of ATP concentration by F0F1-ATP synthase inhibitor oligomycin A renders HeLa cells resistant to the apoptosis induction by treatment with MNNG/ABT-888. Unlike in HeLa cells, ABT-888 protected MNNG induced cell death in normal human fibroblasts. Our study provides evidence that PARP activity determines the fate of HeLa cells by regulating the level of ATP after treatment with MNNG.

Original languageEnglish (US)
Pages (from-to)934-941
Number of pages8
JournalCancer Biology and Therapy
Volume7
Issue number6
StatePublished - Jun 2008

Fingerprint

Nitrosoguanidines
Poly(ADP-ribose) Polymerases
HeLa Cells
DNA Damage
Apoptosis
Adenosine Triphosphate
Cytochromes c
Cell Death
Cytosol
Mitochondria
Necrosis
Methylnitronitrosoguanidine
DNA Breaks
Alkylating Agents
veliparib
Down-Regulation
Fibroblasts

Keywords

  • ABT-888
  • ATP
  • Apoptosis
  • MNNG
  • Necrosis
  • PARP

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Liu, X., Luo, X., Shi, Y., Zhu, G. D., Penning, T., Giranda, V. L., & Luo, Y. (2008). Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage. Cancer Biology and Therapy, 7(6), 934-941.

Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage. / Liu, Xuesong; Luo, Xu; Shi, Yan; Zhu, Gui Dong; Penning, Thomas; Giranda, Vincent L.; Luo, Yan.

In: Cancer Biology and Therapy, Vol. 7, No. 6, 06.2008, p. 934-941.

Research output: Contribution to journalArticle

Liu, X, Luo, X, Shi, Y, Zhu, GD, Penning, T, Giranda, VL & Luo, Y 2008, 'Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage', Cancer Biology and Therapy, vol. 7, no. 6, pp. 934-941.
Liu, Xuesong ; Luo, Xu ; Shi, Yan ; Zhu, Gui Dong ; Penning, Thomas ; Giranda, Vincent L. ; Luo, Yan. / Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage. In: Cancer Biology and Therapy. 2008 ; Vol. 7, No. 6. pp. 934-941.
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