Pollen-mediated gene flow from glyphosate-resistant common waterhemp (Amaranthus rudis Sauer): Consequences for the dispersal of resistance genes

Debalin Sarangi, Andrew J. Tyre, Eric L. Patterson, Todd A. Gaines, Suat Irmak, Stevan Z. Knezevic, John L. Lindquist, Amit J. Jhala

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Abstract

Gene flow is an important component in evolutionary biology; however, the role of gene flow in dispersal of herbicide-resistant alleles among weed populations is poorly understood. Field experiments were conducted at the University of Nebraska-Lincoln to quantify pollen-mediated gene flow (PMGF) from glyphosate-resistant (GR) to -susceptible (GS) common waterhemp using a concentric donor-receptor design. More than 130,000 common waterhemp plants were screened and 26,199 plants were confirmed resistant to glyphosate. Frequency of gene flow from all distances, directions, and years was estimated with a double exponential decay model using Generalized Nonlinear Model (package gnm) in R. PMGF declined by 50% at <3 m distance from the pollen source, whereas 90% reduction was found at 88 m (maximum) depending on the direction of the pollen-receptor blocks. Amplification of the target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), was identified as the mechanism of glyphosate resistance in parent biotype. The EPSPS gene amplification was heritable in common waterhemp and can be transferred via PMGF, and also correlated with glyphosate resistance in pseudo-F 2 progeny. This is the first report of PMGF in GR common waterhemp and the results are critical in explaining the rapid dispersal of GR common waterhemp in Midwestern United States.

Original languageEnglish (US)
Article number44913
JournalScientific reports
Volume7
DOIs
StatePublished - Mar 22 2017

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Amaranthus tuberculatus
glyphosate
gene flow
pollen
genes
gene amplification
receptors
Midwestern United States
nonlinear models
biotypes
herbicides
weeds
deterioration
alleles
Biological Sciences

ASJC Scopus subject areas

  • General

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Pollen-mediated gene flow from glyphosate-resistant common waterhemp (Amaranthus rudis Sauer) : Consequences for the dispersal of resistance genes. / Sarangi, Debalin; Tyre, Andrew J.; Patterson, Eric L.; Gaines, Todd A.; Irmak, Suat; Knezevic, Stevan Z.; Lindquist, John L.; Jhala, Amit J.

In: Scientific reports, Vol. 7, 44913, 22.03.2017.

Research output: Contribution to journalArticle

Sarangi, Debalin ; Tyre, Andrew J. ; Patterson, Eric L. ; Gaines, Todd A. ; Irmak, Suat ; Knezevic, Stevan Z. ; Lindquist, John L. ; Jhala, Amit J. / Pollen-mediated gene flow from glyphosate-resistant common waterhemp (Amaranthus rudis Sauer) : Consequences for the dispersal of resistance genes. In: Scientific reports. 2017 ; Vol. 7.
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abstract = "Gene flow is an important component in evolutionary biology; however, the role of gene flow in dispersal of herbicide-resistant alleles among weed populations is poorly understood. Field experiments were conducted at the University of Nebraska-Lincoln to quantify pollen-mediated gene flow (PMGF) from glyphosate-resistant (GR) to -susceptible (GS) common waterhemp using a concentric donor-receptor design. More than 130,000 common waterhemp plants were screened and 26,199 plants were confirmed resistant to glyphosate. Frequency of gene flow from all distances, directions, and years was estimated with a double exponential decay model using Generalized Nonlinear Model (package gnm) in R. PMGF declined by 50{\%} at <3 m distance from the pollen source, whereas 90{\%} reduction was found at 88 m (maximum) depending on the direction of the pollen-receptor blocks. Amplification of the target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), was identified as the mechanism of glyphosate resistance in parent biotype. The EPSPS gene amplification was heritable in common waterhemp and can be transferred via PMGF, and also correlated with glyphosate resistance in pseudo-F 2 progeny. This is the first report of PMGF in GR common waterhemp and the results are critical in explaining the rapid dispersal of GR common waterhemp in Midwestern United States.",
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