Polarized secretion of transgene products from salivary glands in vivo

Bruce J. Baum, Mark E. Berkman, Yitzhak Marmary, Corinne M. Goldsmith, Lorena Baccaglini, Songlin Wang, Robert B. Wellner, A. T.M.Shamsul Hoque, Jane C. Atkinson, Hisako Yamagishi, Hideaki Kagami, Albert F. Parlow, Julie Chao

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Previously (Kagami et al. Hum. Gene Ther. 1996;7:2177-2184) we have shown that salivary glands are able to secrete a transgene-encoded protein into serum as well as saliva. This result and other published data suggest that salivary glands may be a useful target site for vectors encoding therapeutic proteins for systemic delivery. The aim of the present study was to assess in vivo if transgene-encoded secretory proteins follow distinct, polarized sorting pathways as has been shown to occur 'classically' in cell biological studies in vitro. Four first-generation, E1-, type 5 recombinant adenoviruses were used to deliver different transgenes to a rat submandibular cell line in vitro or to rat submandibular glands in vivo. Subsequently, the secretory distribution of the encoded proteins was determined. Luciferase, which has no signal peptide, served as a cell-associated, negative control and was used to correct for any nonspecific secretory protein release from cells. The three remaining transgene products tested, human tissue kallikrein (hK1), human growth hormone (hGH), and human α1-antitrypsin (hα1AT), were predominantly secreted (>96%) in vitro. Most importantly, in vivo, after a parasympathomimetic secretory stimulus, both hK1 and hGH were secreted primarily in an exocrine manner into saliva. Conversely, hα1AT was predominantly secreted into the bloodstream, i.e., in an endocrine manner. The aggregate results are consistent with the recognition of signals encoded within the transgenes that result in specific patterns of polarized protein secretion from rat submandibular gland cells in vivo.

Original languageEnglish (US)
Pages (from-to)2789-2797
Number of pages9
JournalHuman gene therapy
Volume10
Issue number17
DOIs
StatePublished - Nov 20 1999

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Salivary Glands
Transgenes
Human Growth Hormone
Submandibular Gland
Proteins
Saliva
Parasympathomimetics
Tissue Kallikreins
Protein Sorting Signals
Luciferases
Adenoviridae
Blood Proteins
Cell Line
Genes
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Baum, B. J., Berkman, M. E., Marmary, Y., Goldsmith, C. M., Baccaglini, L., Wang, S., ... Chao, J. (1999). Polarized secretion of transgene products from salivary glands in vivo. Human gene therapy, 10(17), 2789-2797. https://doi.org/10.1089/10430349950016528

Polarized secretion of transgene products from salivary glands in vivo. / Baum, Bruce J.; Berkman, Mark E.; Marmary, Yitzhak; Goldsmith, Corinne M.; Baccaglini, Lorena; Wang, Songlin; Wellner, Robert B.; Hoque, A. T.M.Shamsul; Atkinson, Jane C.; Yamagishi, Hisako; Kagami, Hideaki; Parlow, Albert F.; Chao, Julie.

In: Human gene therapy, Vol. 10, No. 17, 20.11.1999, p. 2789-2797.

Research output: Contribution to journalArticle

Baum, BJ, Berkman, ME, Marmary, Y, Goldsmith, CM, Baccaglini, L, Wang, S, Wellner, RB, Hoque, ATMS, Atkinson, JC, Yamagishi, H, Kagami, H, Parlow, AF & Chao, J 1999, 'Polarized secretion of transgene products from salivary glands in vivo', Human gene therapy, vol. 10, no. 17, pp. 2789-2797. https://doi.org/10.1089/10430349950016528
Baum, Bruce J. ; Berkman, Mark E. ; Marmary, Yitzhak ; Goldsmith, Corinne M. ; Baccaglini, Lorena ; Wang, Songlin ; Wellner, Robert B. ; Hoque, A. T.M.Shamsul ; Atkinson, Jane C. ; Yamagishi, Hisako ; Kagami, Hideaki ; Parlow, Albert F. ; Chao, Julie. / Polarized secretion of transgene products from salivary glands in vivo. In: Human gene therapy. 1999 ; Vol. 10, No. 17. pp. 2789-2797.
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