Pneumatic microfluidic cell compression device for high-throughput study of chondrocyte mechanobiology

Donghee Lee, Alek Erickson, Taesun You, Andrew T Dudley, Sangjin Ryu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Hyaline cartilage is a specialized type of connective tissue that lines many moveable joints (articular cartilage) and contributes to bone growth (growth plate cartilage). Hyaline cartilage is composed of a single cell type, the chondrocyte, which produces a unique hydrated matrix to resist compressive stress. Although compressive stress has profound effects on transcriptional networks and matrix biosynthesis in chondrocytes, mechanistic relationships between strain, signal transduction, cell metabolism, and matrix production remain superficial. Here, we describe development and validation of a polydimethylsiloxane (PDMS)-based pneumatic microfluidic cell compression device which generates multiple compression conditions in a single platform. The device contained an array of PDMS balloons of different sizes which were actuated by pressurized air, and the balloons compressed chondrocytes cells in alginate hydrogel constructs. Our characterization and testing of the device showed that the developed platform could compress chondrocytes with various magnitudes simultaneously with negligible effect on cell viability. Also, the device is compatible with live cell imaging to probe early effects of compressive stress, and it can be rapidly dismantled to facilitate molecular studies of compressive stress on transcriptional networks. Therefore, the proposed device will enhance the productivity of chondrocyte mechanobiology studies, and it can be applied to study mechanobiology of other cell types.

Original languageEnglish (US)
Pages (from-to)2077-2086
Number of pages10
JournalLab on a Chip
Volume18
Issue number14
DOIs
StatePublished - Jan 1 2018

Fingerprint

Biophysics
Microfluidics
Cartilage
Chondrocytes
Compressive stress
Pneumatics
Throughput
Equipment and Supplies
Balloons
Polydimethylsiloxane
Hyaline Cartilage
Gene Regulatory Networks
Signal transduction
Hydrogel
Alginate
Biosynthesis
Metabolism
Hydrogels
Compressed Air
Bone

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Pneumatic microfluidic cell compression device for high-throughput study of chondrocyte mechanobiology. / Lee, Donghee; Erickson, Alek; You, Taesun; Dudley, Andrew T; Ryu, Sangjin.

In: Lab on a Chip, Vol. 18, No. 14, 01.01.2018, p. 2077-2086.

Research output: Contribution to journalArticle

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