Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation

Kelly E. Roney, Brian P. O'Connor, Haitao Wen, Eda K. Holl, Elizabeth H. Guthrie, Beckley K. Davis, Stephen W. Jones, Sushmita Jha, Lisa Sharek, Rafael Garcia-Mata, James E. Bear, Jenny P.Y. Ting

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Plexins are cell surface receptors widely studied in the nervous system, where they mediate migration and morphogenesis though the Rho family of small GTPases. More recently, plexins have been implicated in immune processes including cell-cell interaction, immune activation, migration, and cytokine production. Plexin-B2 facilitates ligand induced cell guidance and migration in the nervous system, and induces cytoskeletal changes in overexpression assays through RhoGTPase. The function of Plexin-B2 in the immune system is unknown. This report shows that Plexin-B2 is highly expressed on cells of the innate immune system in the mouse, including macrophages, conventional dendritic cells, and plasmacytoid dendritic cells. However, Plexin-B2 does not appear to regulate the production of proinflammatory cytokines, phagocytosis of a variety of targets, or directional migration towards chemoattractants or extracellular matrix in mouse macrophages. Instead, Plxnb2 -/- macrophages have greater cellular motility than wild type in the unstimulated state that is accompanied by more active, GTP-bound Rac and Cdc42. Additionally, Plxnb2 -/- macrophages demonstrate faster in vitro wound closure activity. Studies have shown that a closely related family member, Plexin-B1, binds to active Rac and sequesters it from downstream signaling. The interaction of Plexin-B2 with Rac has only been previously confirmed in yeast and bacterial overexpression assays. The data presented here show that Plexin-B2 functions in mouse macrophages as a negative regulator of the GTPases Rac and Cdc42 and as a negative regulator of basal cell motility and wound healing.

Original languageEnglish (US)
Article numbere24795
JournalPloS one
Volume6
Issue number9
DOIs
StatePublished - Sep 23 2011

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Macrophages
macrophages
Chemical activation
guanosinetriphosphatase
dendritic cells
cell movement
nervous system
cells
mice
Immune system
cytokines
Neurology
Dendritic Cells
Nervous System
Cell Movement
Immune System
Assays
chemoattractants
assays
tissue repair

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Roney, K. E., O'Connor, B. P., Wen, H., Holl, E. K., Guthrie, E. H., Davis, B. K., ... Ting, J. P. Y. (2011). Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. PloS one, 6(9), [e24795]. https://doi.org/10.1371/journal.pone.0024795

Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. / Roney, Kelly E.; O'Connor, Brian P.; Wen, Haitao; Holl, Eda K.; Guthrie, Elizabeth H.; Davis, Beckley K.; Jones, Stephen W.; Jha, Sushmita; Sharek, Lisa; Garcia-Mata, Rafael; Bear, James E.; Ting, Jenny P.Y.

In: PloS one, Vol. 6, No. 9, e24795, 23.09.2011.

Research output: Contribution to journalArticle

Roney, KE, O'Connor, BP, Wen, H, Holl, EK, Guthrie, EH, Davis, BK, Jones, SW, Jha, S, Sharek, L, Garcia-Mata, R, Bear, JE & Ting, JPY 2011, 'Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation', PloS one, vol. 6, no. 9, e24795. https://doi.org/10.1371/journal.pone.0024795
Roney KE, O'Connor BP, Wen H, Holl EK, Guthrie EH, Davis BK et al. Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. PloS one. 2011 Sep 23;6(9). e24795. https://doi.org/10.1371/journal.pone.0024795
Roney, Kelly E. ; O'Connor, Brian P. ; Wen, Haitao ; Holl, Eda K. ; Guthrie, Elizabeth H. ; Davis, Beckley K. ; Jones, Stephen W. ; Jha, Sushmita ; Sharek, Lisa ; Garcia-Mata, Rafael ; Bear, James E. ; Ting, Jenny P.Y. / Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. In: PloS one. 2011 ; Vol. 6, No. 9.
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