Platelet membrane glycoproteins IIb and IIIa are substrates of purified pp60c-src protein tyrosine kinase

Duygu Findik, Christoph Reuter, Peter Presek

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Human platelet glycoproteins IIb and IIIa form the receptor for fibrinogen, von Willebrand factor and fibronectin. Isolated human glycoproteins IIb-IIIa are phosphorylated by purified pp60c-src protein tyrosine kinase. Analysis of the phosphorylated proteins on SDS-PAGE showed that under reducing conditions both phosphoproteins change their relative molecular masses from 135 to 120 kDa and from 97 to 105 kDa, which are characteristic properties of glycoproteins IIb-IIIa. Phosphorylated proteins could be immunoprecipitated with an antiserum against glycoproteins IIb-IIIa but not by control serum. Some kinetic properties of the glycoprotein phosphorylations are also investigated. How the glycoprotein IIb-IIIa complex acquires its receptor activity in stimulated platelets is unknown; however, phosphorylation could be an important mechanism.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalFEBS Letters
Volume262
Issue number1
DOIs
StatePublished - Mar 12 1990

Fingerprint

Platelet Membrane Glycoprotein IIb
Integrin beta3
Platelet Glycoprotein GPIIb-IIIa Complex
src-Family Kinases
Substrates
Phosphorylation
Fibrinogen Receptors
Platelet Membrane Glycoproteins
Phosphoproteins
von Willebrand Factor
Molecular mass
Platelets
Fibronectins
Immune Sera
Polyacrylamide Gel Electrophoresis
Glycoproteins
Proteins
Blood Platelets
Kinetics
Serum

Keywords

  • (Platelet)
  • Glycoprotein IIIa
  • Glycoprotein IIb
  • Integrin
  • Protein tyrosine kinase
  • ppo60

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Platelet membrane glycoproteins IIb and IIIa are substrates of purified pp60c-src protein tyrosine kinase. / Findik, Duygu; Reuter, Christoph; Presek, Peter.

In: FEBS Letters, Vol. 262, No. 1, 12.03.1990, p. 1-4.

Research output: Contribution to journalArticle

Findik, Duygu ; Reuter, Christoph ; Presek, Peter. / Platelet membrane glycoproteins IIb and IIIa are substrates of purified pp60c-src protein tyrosine kinase. In: FEBS Letters. 1990 ; Vol. 262, No. 1. pp. 1-4.
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