Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2

R. N.N. Han, S. Buch, B. A. Freeman, M. Post, A. K. Tanswell

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Abstract

The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.

Original languageEnglish (US)
Pages (from-to)L140-L146
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume262
Issue number2 6-2
Publication statusPublished - Jan 1 1992

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Keywords

  • platelet-derived growth factor receptor
  • pulmonary fibrosis
  • pulmonary oxygen toxicity

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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