Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2

R. N N Han, Shilpa J Buch, B. A. Freeman, M. Post, A. K. Tanswell

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume262
Issue number2 6-2
StatePublished - Jan 1 1992

Fingerprint

Proto-Oncogene Proteins c-sis
Platelet-Derived Growth Factor
Lung
Platelet-Derived Growth Factor Receptors
Messenger RNA
Growth
Genes
Cell Division
Gene Expression
Autoradiography
Hyperplasia
Fibroblasts
Western Blotting
Immunohistochemistry
Air

Keywords

  • platelet-derived growth factor receptor
  • pulmonary fibrosis
  • pulmonary oxygen toxicity

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2. / Han, R. N N; Buch, Shilpa J; Freeman, B. A.; Post, M.; Tanswell, A. K.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 262, No. 2 6-2, 01.01.1992.

Research output: Contribution to journalArticle

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