Platelet-derived growth factor and growth-related genes in rat lung. I. Developmental expression.

S. Buch, C. Jones, N. Sweezey, K. Tanswell, M. Post

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The autocrine, paracrine, or systemic growth factors responsible for fetal lung cell growth are not completely defined. The progression-type insulin-like growth factors and epidermal growth factor, or transforming growth factor-alpha acting through the epidermal growth factor receptor, appear to act on the developing lung epithelium. The competence factors that facilitate the actions of progression factors during lung growth are unknown. Fetal rat lung cells in vitro synthesize a platelet-derived growth factor (PDGF)-like polypeptide, which we have hypothesized may play a paracrine role in normal lung development. Slot blot and Northern blot analyses of fetal rat lung mRNA have been used to determine if there is a relationship between expression of message for PDGF-A or PDGF-B chains, or their cognate receptors, and periods of maximal growth during late fetal rat lung development. Whole lung mRNA was extracted on 18, 19, 20, 21, and 22 days of gestation (term = 22 days). The peak of DNA synthesis, as assessed by expression of message for DNA polymerase alpha, histone 3, and the proto-oncogenes c-fos and c-myc, which are stimulated by binding of growth factors including PDGF, occurred during the canalicular stage of lung development on days 19 and 20 of gestation. Expression of message for PDGF-A and PDGF-B chains was low during the pseudoglandular stage on day 18, peaked during the canalicular stage on days 19 and 20, then fell again during the saccular stage at days 21 and 22 of gestation.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)371-376
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume5
Issue number4
DOIs
StatePublished - Oct 1991

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Platelet-Derived Growth Factor
Proto-Oncogene Proteins c-sis
Rats
Genes
Lung
Intercellular Signaling Peptides and Proteins
Growth
DNA Polymerase I
Messenger RNA
Transforming Growth Factor alpha
Cell growth
Somatomedins
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Histones
Pregnancy
Peptides
DNA
fos Genes
Northern Blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Platelet-derived growth factor and growth-related genes in rat lung. I. Developmental expression. / Buch, S.; Jones, C.; Sweezey, N.; Tanswell, K.; Post, M.

In: American journal of respiratory cell and molecular biology, Vol. 5, No. 4, 10.1991, p. 371-376.

Research output: Contribution to journalArticle

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