Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid

Jocelyn F. Krey, Evan S. Krystofiak, Rachel A. Dumont, Sarath Vijayakumar, Dongseok Choi, Francisco Rivero, Bechara Kachar, Sherri M Jones, Peter G. Barr-Gillespie

Research output: Contribution to journalArticle

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Abstract

With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either lacked plastin 1 (PLS1) or had nonfunctional fascin 2 (FSCN2) had reduced inner ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant cross-linker in vestibular stereocilia and the second most abundant protein overall; ESPN only accounted for ~15% of the total cross-linkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, although wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Although all three cross-linkers are required for stereocilia structure and function, PLS1 biases actin toward liquid packing, which allows stereocilia to grow to a greater diameter.

Original languageEnglish (US)
Pages (from-to)467-482
Number of pages16
JournalJournal of Cell Biology
Volume215
Issue number4
DOIs
StatePublished - Jan 1 2016

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Stereocilia
Actin Cytoskeleton
Actins
Inner Ear
Cell Surface Extensions
Saccule and Utricle
plastin
Mass Spectrometry

ASJC Scopus subject areas

  • Cell Biology

Cite this

Krey, J. F., Krystofiak, E. S., Dumont, R. A., Vijayakumar, S., Choi, D., Rivero, F., ... Barr-Gillespie, P. G. (2016). Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. Journal of Cell Biology, 215(4), 467-482. https://doi.org/10.1083/jcb.201606036

Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. / Krey, Jocelyn F.; Krystofiak, Evan S.; Dumont, Rachel A.; Vijayakumar, Sarath; Choi, Dongseok; Rivero, Francisco; Kachar, Bechara; Jones, Sherri M; Barr-Gillespie, Peter G.

In: Journal of Cell Biology, Vol. 215, No. 4, 01.01.2016, p. 467-482.

Research output: Contribution to journalArticle

Krey, JF, Krystofiak, ES, Dumont, RA, Vijayakumar, S, Choi, D, Rivero, F, Kachar, B, Jones, SM & Barr-Gillespie, PG 2016, 'Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid', Journal of Cell Biology, vol. 215, no. 4, pp. 467-482. https://doi.org/10.1083/jcb.201606036
Krey, Jocelyn F. ; Krystofiak, Evan S. ; Dumont, Rachel A. ; Vijayakumar, Sarath ; Choi, Dongseok ; Rivero, Francisco ; Kachar, Bechara ; Jones, Sherri M ; Barr-Gillespie, Peter G. / Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. In: Journal of Cell Biology. 2016 ; Vol. 215, No. 4. pp. 467-482.
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