Plasmodium falciparum in vitro drug resistance selections and gene editing

Caroline L Ng, David A. Fidock

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Malaria continues to be a global health burden, threatening over 40% of the world’s population. Drug resistance in Plasmodium falciparum, the etiological agent of the majority of human malaria cases, is compromising elimination efforts. New approaches to treating drug-resistant malaria benefit from defining resistance liabilities of known antimalarial agents and compounds in development and defining genetic changes that mediate loss of parasite susceptibility. Here, we present protocols for in vitro selection of drug-resistant parasites and for site-directed gene editing of candidate resistance mediators to test for causality.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-140
Number of pages18
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2013
ISSN (Print)1064-3745

Fingerprint

Plasmodium falciparum
Drug Resistance
Malaria
Parasites
Antimalarials
Causality
Pharmaceutical Preparations
Population
Gene Editing
In Vitro Techniques

Keywords

  • CRISPR/Cas9
  • Drug resistance
  • Gene editing
  • Malaria
  • Plasmodium falciparum
  • Selections
  • ZFN

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ng, C. L., & Fidock, D. A. (2019). Plasmodium falciparum in vitro drug resistance selections and gene editing. In Methods in Molecular Biology (pp. 123-140). (Methods in Molecular Biology; Vol. 2013). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9550-9_9

Plasmodium falciparum in vitro drug resistance selections and gene editing. / Ng, Caroline L; Fidock, David A.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 123-140 (Methods in Molecular Biology; Vol. 2013).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ng, CL & Fidock, DA 2019, Plasmodium falciparum in vitro drug resistance selections and gene editing. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2013, Humana Press Inc., pp. 123-140. https://doi.org/10.1007/978-1-4939-9550-9_9
Ng CL, Fidock DA. Plasmodium falciparum in vitro drug resistance selections and gene editing. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 123-140. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9550-9_9
Ng, Caroline L ; Fidock, David A. / Plasmodium falciparum in vitro drug resistance selections and gene editing. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 123-140 (Methods in Molecular Biology).
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