Abstract

Lentiviral replication in its target cells affects a delicate balance between cellular cofactors required for virus propagation and immunoregulation for host defense. To better elucidate cellular proteins linked to viral infection, we tested plasma from rhesus macaques infected with the simian immunodeficiency viral strain SIVsmm9, prior to, 10 days (acute), and 49 weeks (chronic) after viral infection. Changes in plasma protein content were measured by quantitative mass spectrometry by isobaric tags for absolute and relative quantitation (iTRAQ) methods. An 81 and 232% increase in SERPINA1 was seen during acute and chronic infection, respectively. Interestingly, gelsolin, vitamin D binding protein and histidine rich glycoprotein were decreased by 45% in acute conditions but returned to baseline during chronic infection. When compared to uninfected controls, a 48-103% increase in leucine rich alpha 2-glycoprotein, vitronectin, and ceruloplasmin was observed during chronic viral infection. Observed changes in plasma proteins expression likely represent a compensatory host response to persistent viral infection.

Original languageEnglish (US)
Pages (from-to)4721-4731
Number of pages11
JournalJournal of proteome research
Volume9
Issue number9
DOIs
StatePublished - Sep 3 2010

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Simian Immunodeficiency Virus
Virus Diseases
Macaca mulatta
Viruses
Proteomics
Blood Proteins
Vitamin D-Binding Protein
Gelsolin
Vitronectin
Plasmas
Ceruloplasmin
Leucine
Mass spectrometry
Glycoproteins
Infection
Mass Spectrometry
Proteins
histidine-rich proteins

Keywords

  • biomarker
  • iTRAQ
  • monkey
  • plasma
  • proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Plasma proteomic analysis of simian immunodeficiency virus infection of rhesus macaques. / Wiederin, Jayme L.; Donahoe, Robert M.; Anderson, James R.; Yu, Fang; Fox, Howard S; Gendelman, Howard Eliot; Ciborowski, Pawel S.

In: Journal of proteome research, Vol. 9, No. 9, 03.09.2010, p. 4721-4731.

Research output: Contribution to journalArticle

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