Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae

Jessie Fernandez, Margarita Marroquin-Guzman, Renu Nandakumar, Sara Shijo, Kathryn M. Cornwell, Gang Li, Richard A. Wilson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Crop destruction by the hemibiotrophic rice pathogen Magnaporthe oryzae requires plant defence suppression to facilitate extensive biotrophic growth in host cells before the onset of necrosis. How this is achieved at the genetic level is not well understood. Here, we report that a M. oryzae sirtuin, MoSir2, plays an essential role in rice defence suppression and colonization by controlling superoxide dismutase (SOD) gene expression. Loss of MoSir2 function in Δsir2 strains did not affect appressorial function, but biotrophic growth in rice cells was attenuated. Compared to wild type, Δsir2 strains failed to neutralize plant-derived reactive oxygen species (ROS) and elicited robust defence responses in rice epidermal cells that included elevated pathogenesis-related gene expression and granular depositions. Deletion of a SOD-encoding gene under MoSir2 control generated Δsod1 deletion strains that mimicked Δsir2 for impaired rice defence suppression, confirming SOD activity as a downstream output of MoSir2. In addition, comparative protein acetylation studies and forward genetic analyses identified a JmjC domain-containing protein as a likely target of MoSir2, and a Δsir2 Δjmjc double mutant was restored for MoSOD1 expression and defence suppression in rice epidermal cells. Together, this work reveals MoSir2 and MoJmjC as novel regulators of early rice cell infection.

Original languageEnglish (US)
Pages (from-to)70-88
Number of pages19
JournalMolecular Microbiology
Volume94
Issue number1
DOIs
StatePublished - Oct 1 2014

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Magnaporthe
Infection
Superoxide Dismutase
Gene Expression
Oryza
Acetylation
Growth
Reactive Oxygen Species
Necrosis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae. / Fernandez, Jessie; Marroquin-Guzman, Margarita; Nandakumar, Renu; Shijo, Sara; Cornwell, Kathryn M.; Li, Gang; Wilson, Richard A.

In: Molecular Microbiology, Vol. 94, No. 1, 01.10.2014, p. 70-88.

Research output: Contribution to journalArticle

Fernandez, J, Marroquin-Guzman, M, Nandakumar, R, Shijo, S, Cornwell, KM, Li, G & Wilson, RA 2014, 'Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae', Molecular Microbiology, vol. 94, no. 1, pp. 70-88. https://doi.org/10.1111/mmi.12743
Fernandez J, Marroquin-Guzman M, Nandakumar R, Shijo S, Cornwell KM, Li G et al. Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae. Molecular Microbiology. 2014 Oct 1;94(1):70-88. https://doi.org/10.1111/mmi.12743
Fernandez, Jessie ; Marroquin-Guzman, Margarita ; Nandakumar, Renu ; Shijo, Sara ; Cornwell, Kathryn M. ; Li, Gang ; Wilson, Richard A. / Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae. In: Molecular Microbiology. 2014 ; Vol. 94, No. 1. pp. 70-88.
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