Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: Identification of unique and shared domains

James K. Wahl, Paula A. Sacco, Tammy M. McGranahan-Sadler, Laura M. Sauppé, Margaret J. Wheelock, Keith R. Johnson

Research output: Contribution to journalArticle

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Abstract

Two cell-cell junctions, the adherens junction and the desmosome, are prominent in epithelial cells. These junctions are composed of transmembrane cadherins which interact with cytoplasmic proteins that serve to link the cadherin to the cytoskeleton. One component of both adherens junctions and desmosomes is plakoglobin. In the adherens junction plakoglobin interacts with both the classical cadherin and with α-catenin. Alpha-catenin in turn interacts with microfilaments. The role plakoglobin plays in the desmosome is not well understood. Plakoglobin interacts with the desmosomal cadherins, but how and if this mediates interactions with the intermediate filament cytoskeleton is not known. Here we compare the domains of plakoglobin that allow it to associate with the desmosomal cadherins with those involved in interactions with the classical cadherins. We show that three sites on plakoglobin are involved in associations with the desmosomal cadherins. A domain near the N terminus is unique to the desmosomal cadherins and overlaps with the site that interacts with α-catenin, suggesting that there may be competition between α-catenin and the desmosomal cadherins for interactions with plakoglobin. In addition, a central domain is shared with regions used by plakoglobin to associate with the classical cadherins. Finally, a domain near the C terminus is shown to strongly modulate the interactions with the desmosomal cadherins. This latter domain also contributes to the association of plakoglobin with the classical cadherins.

Original languageEnglish (US)
Pages (from-to)1143-1154
Number of pages12
JournalJournal of cell science
Volume109
Issue number5
StatePublished - Jun 14 1996

Fingerprint

Desmosomal Cadherins
gamma Catenin
Cadherins
Adherens Junctions
Catenins
Desmosomes
Cytoskeleton
alpha Catenin
Intercellular Junctions
Intermediate Filaments
Actin Cytoskeleton
Epithelial Cells

Keywords

  • Adherens junction
  • Cadherin
  • Desmosome
  • Plakoglobin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins : Identification of unique and shared domains. / Wahl, James K.; Sacco, Paula A.; McGranahan-Sadler, Tammy M.; Sauppé, Laura M.; Wheelock, Margaret J.; Johnson, Keith R.

In: Journal of cell science, Vol. 109, No. 5, 14.06.1996, p. 1143-1154.

Research output: Contribution to journalArticle

Wahl, James K. ; Sacco, Paula A. ; McGranahan-Sadler, Tammy M. ; Sauppé, Laura M. ; Wheelock, Margaret J. ; Johnson, Keith R. / Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins : Identification of unique and shared domains. In: Journal of cell science. 1996 ; Vol. 109, No. 5. pp. 1143-1154.
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