Pilus distribution among lineages of Group B Streptococcus: An evolutionary and clinical perspective

Amber Cody Springman, David W. Lacher, Emily A. Waymire, Samantha L. Wengert, Pallavi Singh, Ruth N. Zadoks, Herbert Dele Davies, Shannon D. Manning

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Group B Streptococcus (GBS) is an opportunistic pathogen in both humans and bovines. Epidemiological and phylogenetic analyses have found strains belonging to certain phylogenetic lineages to be more frequently associated with invasive newborn disease, asymptomatic maternal colonization, and subclinical bovine mastitis. Pilus structures in GBS facilitate colonization and invasion of host tissues and play a role in biofilm formation, though few large-scale studies have estimated the frequency and diversity of the three pilus islands (PIs) across diverse genotypes. Here, we examined the distribution of pilus islands (PI) 1, 2a and 2b among 295 GBS strains representing 73 multilocus sequence types (STs) belonging to eight clonal complexes. PCR-based RFLP was also used to evaluate variation in the genes encoding pilus backbone proteins of PI-2a and PI-2b. Results: All 295 strains harbored one of the PI-2 variants and most human-derived strains contained PI-1. Bovine-derived strains lacked PI-1 and possessed a unique PI-2b backbone protein allele. Neonatal strains more frequently had PI-1 and a PI-2 variant than maternal colonizing strains, and most CC-17 strains had PI-1 and PI-2b with a distinct backbone protein allele. Furthermore, we present evidence for the frequent gain and loss of genes encoding certain pilus types. Conclusions: These data suggest that pilus combinations impact host specificity and disease presentation and that diversification often involves the loss or acquisition of PIs. Such findings have implications for the development of GBS vaccines that target the three pilus islands.

Original languageEnglish (US)
Article number159
JournalBMC microbiology
Volume14
Issue number1
DOIs
StatePublished - Jun 19 2014

Fingerprint

Streptococcus agalactiae
Islands
Alleles
Mothers
Bovine Mastitis
Asymptomatic Diseases
Proteins
Host Specificity
Biofilms
Restriction Fragment Length Polymorphisms
Genes

Keywords

  • MLST
  • Molecular epidemiology
  • Pilus
  • Streptococcus agalactiae

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Springman, A. C., Lacher, D. W., Waymire, E. A., Wengert, S. L., Singh, P., Zadoks, R. N., ... Manning, S. D. (2014). Pilus distribution among lineages of Group B Streptococcus: An evolutionary and clinical perspective. BMC microbiology, 14(1), [159]. https://doi.org/10.1186/1471-2180-14-159

Pilus distribution among lineages of Group B Streptococcus : An evolutionary and clinical perspective. / Springman, Amber Cody; Lacher, David W.; Waymire, Emily A.; Wengert, Samantha L.; Singh, Pallavi; Zadoks, Ruth N.; Davies, Herbert Dele; Manning, Shannon D.

In: BMC microbiology, Vol. 14, No. 1, 159, 19.06.2014.

Research output: Contribution to journalArticle

Springman, AC, Lacher, DW, Waymire, EA, Wengert, SL, Singh, P, Zadoks, RN, Davies, HD & Manning, SD 2014, 'Pilus distribution among lineages of Group B Streptococcus: An evolutionary and clinical perspective', BMC microbiology, vol. 14, no. 1, 159. https://doi.org/10.1186/1471-2180-14-159
Springman, Amber Cody ; Lacher, David W. ; Waymire, Emily A. ; Wengert, Samantha L. ; Singh, Pallavi ; Zadoks, Ruth N. ; Davies, Herbert Dele ; Manning, Shannon D. / Pilus distribution among lineages of Group B Streptococcus : An evolutionary and clinical perspective. In: BMC microbiology. 2014 ; Vol. 14, No. 1.
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AB - Background: Group B Streptococcus (GBS) is an opportunistic pathogen in both humans and bovines. Epidemiological and phylogenetic analyses have found strains belonging to certain phylogenetic lineages to be more frequently associated with invasive newborn disease, asymptomatic maternal colonization, and subclinical bovine mastitis. Pilus structures in GBS facilitate colonization and invasion of host tissues and play a role in biofilm formation, though few large-scale studies have estimated the frequency and diversity of the three pilus islands (PIs) across diverse genotypes. Here, we examined the distribution of pilus islands (PI) 1, 2a and 2b among 295 GBS strains representing 73 multilocus sequence types (STs) belonging to eight clonal complexes. PCR-based RFLP was also used to evaluate variation in the genes encoding pilus backbone proteins of PI-2a and PI-2b. Results: All 295 strains harbored one of the PI-2 variants and most human-derived strains contained PI-1. Bovine-derived strains lacked PI-1 and possessed a unique PI-2b backbone protein allele. Neonatal strains more frequently had PI-1 and a PI-2 variant than maternal colonizing strains, and most CC-17 strains had PI-1 and PI-2b with a distinct backbone protein allele. Furthermore, we present evidence for the frequent gain and loss of genes encoding certain pilus types. Conclusions: These data suggest that pilus combinations impact host specificity and disease presentation and that diversification often involves the loss or acquisition of PIs. Such findings have implications for the development of GBS vaccines that target the three pilus islands.

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