PilG is involved in the regulation of twitching motility and antifungal antibiotic biosynthesis in the biological control agent lysobacter enzymogenes

Xue Zhou, Guoliang Qian, Yuan Chen, Liangcheng Du, Fengquan Liu, Gary Y. Yuen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Lysobacter enzymogenes strain C3 is a gliding bacterium which produces the antifungal secondary metabolite heat-stable antifungal factor (HSAF) and type IV pilus (T4P) as important mechanisms in biological control activity against fungal pathogens. To date, the regulators that control HSAF biosynthesis and T4P-dependent twitching motility in L. enzymogenes are poorly explored. In the present study, we addressed the role of pilG in the regulation of these two traits in L. enzymogenes. PilG of L. enzymogenes was found to be a response regulator, commonly known as a component of a two-component transduction system. Mutation of pilG in strain C3 abolished its ability to display spreading colony phenotype and cell movement at the colony margin, which is indicative of twitching motility; hence, PilG positively regulates twitching motility in L. enzymogenes. Mutation of pilG also enhanced HSAF production and the transcription of its key biosynthetic gene hsaf pks/nrps, suggesting that PilG plays a negative regulatory role in HSAF biosynthesis. This finding represents the first demonstration of the regulator PilG having a role in secondary metabolite biosynthesis in bacteria. Collectively, our results suggest that key ecological functions (HSAF production and twitching motility) in L. enzymogenes strain C3 are regulated in opposite directions by the same regulatory protein, PilG.

Original languageEnglish (US)
Pages (from-to)1318-1324
Number of pages7
JournalPhytopathology
Volume105
Issue number10
DOIs
StatePublished - Oct 1 2015

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Lysobacter enzymogenes
biological control agents
antibiotics
biosynthesis
heat
production economics
secondary metabolites
mutation
fimbriae
ecological function
bacteria
regulatory proteins
cell movement
biological control
transcription (genetics)
phenotype
pathogens

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

PilG is involved in the regulation of twitching motility and antifungal antibiotic biosynthesis in the biological control agent lysobacter enzymogenes. / Zhou, Xue; Qian, Guoliang; Chen, Yuan; Du, Liangcheng; Liu, Fengquan; Yuen, Gary Y.

In: Phytopathology, Vol. 105, No. 10, 01.10.2015, p. 1318-1324.

Research output: Contribution to journalArticle

Zhou, Xue ; Qian, Guoliang ; Chen, Yuan ; Du, Liangcheng ; Liu, Fengquan ; Yuen, Gary Y. / PilG is involved in the regulation of twitching motility and antifungal antibiotic biosynthesis in the biological control agent lysobacter enzymogenes. In: Phytopathology. 2015 ; Vol. 105, No. 10. pp. 1318-1324.
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