Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway

Rituraj Khound, Jing Shen, Yongyan Song, Dipak Santra, Qiaozhu Su

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Scope: This study aims to characterize the effect of fenugreek (Trigonella foenum-graecum) seed and its phytoceutical trigonelline in antimetabolic inflammation and ameliorating overproduction of very low density lipoprotein (VLDL) in insulin resistance. Methods and Results: Two groups of genetic hyperlipidemic mice generated by depletion of cAMP responsive element binding protein H (CREBH) are fed either a chow containing 2% fenugreek seed or vehicle for 7 weeks. Q-RT-PCR and immunoblotting analysis demonstrated that fenugreek seed containing diet inhibits hepatic SREBP-1c activation and the subsequent de novo lipogenesis by enhancing expression of insulin-inducible gene-1 (Insig-1) and gene-2 (Insig-2). mRNA expression of PPARα and its target genes that are involved in fatty acid β-oxidation are also upregulated in the fenugreek seed fed-mice which is accompanied by significantly reduced hepatic lipid accumulation and VLDL secretion, improved endoplasmic reticulum (ER) stress, and ameliorated metabolic inflammation. These actions enhance insulin sensitivity and improve hyperlipidemia. In vitro, treating a rat hepatoma cell line, McA-RH7777 (McA), with trigonelline is able to recapitulate the results observed in vivo. Conclusions: This study unveils a novel mechanism of fenugreek seed and trigonelline in countering hepatic VLDL overproduction and insulin resistance by enhancing the Insig signaling pathways and ameliorating metabolic inflammatory stress in the liver.

Original languageEnglish (US)
Article number1700541
JournalMolecular Nutrition and Food Research
Volume62
Issue number5
DOIs
StatePublished - Mar 2018

Fingerprint

Trigonella
fenugreek
VLDL Lipoproteins
very low density lipoprotein
insulin resistance
Insulin Resistance
trigonelline
Seeds
seeds
liver
Liver
inflammation
Trigonella foenum-graecum
beta oxidation
genes
Sterol Regulatory Element Binding Protein 1
Genes
Inflammation
mice
lipogenesis

Keywords

  • VLDL
  • apoB
  • fenugreek
  • hyperlipidemia
  • insulin resistance
  • trigonelline

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway. / Khound, Rituraj; Shen, Jing; Song, Yongyan; Santra, Dipak; Su, Qiaozhu.

In: Molecular Nutrition and Food Research, Vol. 62, No. 5, 1700541, 03.2018.

Research output: Contribution to journalArticle

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