Physiological and transcriptional memory in guard cells during repetitive dehydration stress

Laetitia Virlouvet, Michael Fromm

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Summary: Arabidopsis plants subjected to a daily dehydration stress and watered recovery cycle display physiological and transcriptional stress memory. Previously stressed plants have stomatal apertures that remain partially closed during a watered recovery period, facilitating reduced transpiration during a subsequent dehydration stress. Guard cells (GCs) display transcriptional memory that is similar to that in leaf tissues for some genes, but display GC-specific transcriptional memory for other genes. The rate-limiting abscisic acid (ABA) biosynthetic genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) and ALDEHYDE OXIDASE 3 (AAO3) are expressed at much higher levels in GCs, particularly during the watered recovery interval, relative to their low levels in leaves. A genetic analysis using mutants in the ABA signaling pathway indicated that GC stomatal memory is ABA-dependent, and that ABA-dependent SNF1-RELATED PROTEIN KINASE 2.2 (SnRK2.2), SnRK2.3 and SnRK2.6 have distinguishable roles in the process. SnRK2.6 is more important for overall stomatal control, while SnRK2.2 and SnRK2.3 are more important for implementing GC stress memory in the subsequent dehydration response. Collectively, our results support a model of altered ABA production in GCs that maintains a partially closed stomatal aperture during an overnight watered recovery period.

Original languageEnglish (US)
Pages (from-to)596-607
Number of pages12
JournalNew Phytologist
Volume205
Issue number2
DOIs
StatePublished - Jan 1 2015

Fingerprint

guard cells
desiccation (plant physiology)
Abscisic Acid
Dehydration
abscisic acid
protein kinases
Genes
Dioxygenases
genes
Arabidopsis
genetic techniques and protocols
leaves
transpiration
mutants

Keywords

  • Abscisic acid (ABA)
  • Arabidopsis thaliana
  • Dehydration stress
  • Guard cell (GC)
  • Memory response
  • SNF1-RELATED PROTEIN KINASE 2 (SnRK2)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Physiological and transcriptional memory in guard cells during repetitive dehydration stress. / Virlouvet, Laetitia; Fromm, Michael.

In: New Phytologist, Vol. 205, No. 2, 01.01.2015, p. 596-607.

Research output: Contribution to journalArticle

Virlouvet, Laetitia ; Fromm, Michael. / Physiological and transcriptional memory in guard cells during repetitive dehydration stress. In: New Phytologist. 2015 ; Vol. 205, No. 2. pp. 596-607.
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