Physiological and behavioral evidence of a capsaicin-sensitive TRPV-like channel in the medicinal leech

Torrie Summers, Sara Holec, Brian D. Burrell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transient receptor potential vanilloid (TRPV) channels are found throughout the animal kingdom, where they play an important role in sensory transduction. In this study, we combined physiological studies with in vivo behavioral experiments to examine the presence of a putative TRPV-like receptor in the medicinal leech, building upon earlier studies in this lophotrochozoan invertebrate. The leech polymodal nociceptive neuron was activated by both peripheral and central application of the TRPV1-activator capsaicin in a concentration-dependent manner, with 100 μmol l-1 being the lowest effective concentration. Responses to capsaicin were inhibited by the selective TRPV1 antagonist SB366791. The polymodal nociceptive neuron also responded to noxious thermal stimuli (>40°C), and this response was also blocked by SB366791. Capsaicin sensitivity was selective to the polymodal nociceptor with no direct response being elicited in the mechanical nociceptive neuron or in the nonnociceptive touch- or pressure-sensitive neurons. Capsaicin also elicited nocifensive behavioral responses (withdrawals and locomotion) in a concentration-dependent manner, and these behavioral responses were significantly attenuated with SB366791. These results suggest the presence of a capsaicin-sensitive TRPVlike channel in the medicinal leech central nervous system and are relevant to the evolution of nociceptive signaling.

Original languageEnglish (US)
Pages (from-to)4167-4173
Number of pages7
JournalJournal of Experimental Biology
Volume217
Issue number23
DOIs
StatePublished - Dec 1 2014

Fingerprint

TRPV Cation Channels
Leeches
leech
capsaicin
Hirudinea
Capsaicin
Nociceptors
behavioral response
receptors
neurons
locomotion
nervous system
Transient Receptor Potential Channels
invertebrate
touch (sensation)
Touch
Invertebrates
Locomotion
animal
central nervous system

Keywords

  • Invertebrate
  • Leech
  • Nociception
  • TRPV

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Cite this

Physiological and behavioral evidence of a capsaicin-sensitive TRPV-like channel in the medicinal leech. / Summers, Torrie; Holec, Sara; Burrell, Brian D.

In: Journal of Experimental Biology, Vol. 217, No. 23, 01.12.2014, p. 4167-4173.

Research output: Contribution to journalArticle

@article{ade80b3f638b40c188a16a0cf3dd24d9,
title = "Physiological and behavioral evidence of a capsaicin-sensitive TRPV-like channel in the medicinal leech",
abstract = "Transient receptor potential vanilloid (TRPV) channels are found throughout the animal kingdom, where they play an important role in sensory transduction. In this study, we combined physiological studies with in vivo behavioral experiments to examine the presence of a putative TRPV-like receptor in the medicinal leech, building upon earlier studies in this lophotrochozoan invertebrate. The leech polymodal nociceptive neuron was activated by both peripheral and central application of the TRPV1-activator capsaicin in a concentration-dependent manner, with 100 μmol l-1 being the lowest effective concentration. Responses to capsaicin were inhibited by the selective TRPV1 antagonist SB366791. The polymodal nociceptive neuron also responded to noxious thermal stimuli (>40°C), and this response was also blocked by SB366791. Capsaicin sensitivity was selective to the polymodal nociceptor with no direct response being elicited in the mechanical nociceptive neuron or in the nonnociceptive touch- or pressure-sensitive neurons. Capsaicin also elicited nocifensive behavioral responses (withdrawals and locomotion) in a concentration-dependent manner, and these behavioral responses were significantly attenuated with SB366791. These results suggest the presence of a capsaicin-sensitive TRPVlike channel in the medicinal leech central nervous system and are relevant to the evolution of nociceptive signaling.",
keywords = "Invertebrate, Leech, Nociception, TRPV",
author = "Torrie Summers and Sara Holec and Burrell, {Brian D.}",
year = "2014",
month = "12",
day = "1",
doi = "10.1242/jeb.110049",
language = "English (US)",
volume = "217",
pages = "4167--4173",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "23",

}

TY - JOUR

T1 - Physiological and behavioral evidence of a capsaicin-sensitive TRPV-like channel in the medicinal leech

AU - Summers, Torrie

AU - Holec, Sara

AU - Burrell, Brian D.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Transient receptor potential vanilloid (TRPV) channels are found throughout the animal kingdom, where they play an important role in sensory transduction. In this study, we combined physiological studies with in vivo behavioral experiments to examine the presence of a putative TRPV-like receptor in the medicinal leech, building upon earlier studies in this lophotrochozoan invertebrate. The leech polymodal nociceptive neuron was activated by both peripheral and central application of the TRPV1-activator capsaicin in a concentration-dependent manner, with 100 μmol l-1 being the lowest effective concentration. Responses to capsaicin were inhibited by the selective TRPV1 antagonist SB366791. The polymodal nociceptive neuron also responded to noxious thermal stimuli (>40°C), and this response was also blocked by SB366791. Capsaicin sensitivity was selective to the polymodal nociceptor with no direct response being elicited in the mechanical nociceptive neuron or in the nonnociceptive touch- or pressure-sensitive neurons. Capsaicin also elicited nocifensive behavioral responses (withdrawals and locomotion) in a concentration-dependent manner, and these behavioral responses were significantly attenuated with SB366791. These results suggest the presence of a capsaicin-sensitive TRPVlike channel in the medicinal leech central nervous system and are relevant to the evolution of nociceptive signaling.

AB - Transient receptor potential vanilloid (TRPV) channels are found throughout the animal kingdom, where they play an important role in sensory transduction. In this study, we combined physiological studies with in vivo behavioral experiments to examine the presence of a putative TRPV-like receptor in the medicinal leech, building upon earlier studies in this lophotrochozoan invertebrate. The leech polymodal nociceptive neuron was activated by both peripheral and central application of the TRPV1-activator capsaicin in a concentration-dependent manner, with 100 μmol l-1 being the lowest effective concentration. Responses to capsaicin were inhibited by the selective TRPV1 antagonist SB366791. The polymodal nociceptive neuron also responded to noxious thermal stimuli (>40°C), and this response was also blocked by SB366791. Capsaicin sensitivity was selective to the polymodal nociceptor with no direct response being elicited in the mechanical nociceptive neuron or in the nonnociceptive touch- or pressure-sensitive neurons. Capsaicin also elicited nocifensive behavioral responses (withdrawals and locomotion) in a concentration-dependent manner, and these behavioral responses were significantly attenuated with SB366791. These results suggest the presence of a capsaicin-sensitive TRPVlike channel in the medicinal leech central nervous system and are relevant to the evolution of nociceptive signaling.

KW - Invertebrate

KW - Leech

KW - Nociception

KW - TRPV

UR - http://www.scopus.com/inward/record.url?scp=84925854641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925854641&partnerID=8YFLogxK

U2 - 10.1242/jeb.110049

DO - 10.1242/jeb.110049

M3 - Article

C2 - 25324339

AN - SCOPUS:84925854641

VL - 217

SP - 4167

EP - 4173

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 23

ER -