Physicochemical characterization of creatine N-methylguanidinium salts

Brandon T. Gufford, Kamaraj Sriraghavan, Nicholas J. Miller, Donald W. Miller, Xiaochen Gu, Jonathan L Vennerstrom, Dennis H. Robinson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Creatine is widely used as a dietary supplement for body builders to enhance athletic performance. As the monohydrate, its low solubility in water and high dose lead to water retention and gastrointestinal discomfort. Hence, alternative creatine derivatives with enhanced water solubility and potential therapeutic advantages have been synthesized. As a zwitterionic compound, creatine can form salts at the N-methyl guanidinium or carboxylic acid functional groups. In this study, we determined the aqueous solubilities and partition coefficients of six N-methyl guanidinium salts of creatine compared to those of creatine monohydrate; two of these were new salts, namely, creatine mesylate and creatine hydrogen maleate. The aqueous solubilities of the salts were significantly more than that of creatine monohydrate with the hydrochloride and mesylate being 38 and 30 times more soluble, respectively. The partition coefficients of the creatine salts were very low indicating their relatively high polarity. Permeabilities of creatine pyruvate, citrate, and hydrochloride in Caco-2 monolayers were compared to that of creatine monohydrate. Aside from the creatine citrate salt form that had reduced permeability, there were no significant differences in permeability characteristics in Caco-2 monolayers. Typical of an amphoteric compound, creatine is least soluble in the pH region near the isoelectric point.

Original languageEnglish (US)
Pages (from-to)240-252
Number of pages13
JournalJournal of Dietary Supplements
Volume7
Issue number3
DOIs
StatePublished - Aug 1 2010

Fingerprint

creatine
Creatine
Salts
salts
water solubility
Solubility
Permeability
permeability
Mesylates
partition coefficients
Guanidine
Citric Acid
citrates
Water
maleates
athletic performance
Athletic Performance
Isoelectric Point
isoelectric point
carboxylic acids

Keywords

  • Creatine monohydrate
  • Creatine salts
  • Log P
  • Permeability
  • Physicochemical properties
  • Solubility
  • Thermal analysis

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Pharmacology (medical)

Cite this

Gufford, B. T., Sriraghavan, K., Miller, N. J., Miller, D. W., Gu, X., Vennerstrom, J. L., & Robinson, D. H. (2010). Physicochemical characterization of creatine N-methylguanidinium salts. Journal of Dietary Supplements, 7(3), 240-252. https://doi.org/10.3109/19390211.2010.491507

Physicochemical characterization of creatine N-methylguanidinium salts. / Gufford, Brandon T.; Sriraghavan, Kamaraj; Miller, Nicholas J.; Miller, Donald W.; Gu, Xiaochen; Vennerstrom, Jonathan L; Robinson, Dennis H.

In: Journal of Dietary Supplements, Vol. 7, No. 3, 01.08.2010, p. 240-252.

Research output: Contribution to journalArticle

Gufford, BT, Sriraghavan, K, Miller, NJ, Miller, DW, Gu, X, Vennerstrom, JL & Robinson, DH 2010, 'Physicochemical characterization of creatine N-methylguanidinium salts', Journal of Dietary Supplements, vol. 7, no. 3, pp. 240-252. https://doi.org/10.3109/19390211.2010.491507
Gufford, Brandon T. ; Sriraghavan, Kamaraj ; Miller, Nicholas J. ; Miller, Donald W. ; Gu, Xiaochen ; Vennerstrom, Jonathan L ; Robinson, Dennis H. / Physicochemical characterization of creatine N-methylguanidinium salts. In: Journal of Dietary Supplements. 2010 ; Vol. 7, No. 3. pp. 240-252.
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