Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik, Thomas J Morris, L. G. Weathers, B. F. Rodorf, D. R. Kearns

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalVirology
Volume63
Issue number1
DOIs
StatePublished - Jan 1 1975

Fingerprint

Viroids
Uridine Monophosphate
Cytidine Monophosphate
RNA
Adenosine Monophosphate
Transfer RNA
Digestion
Magnetic Resonance Spectroscopy
Nucleotides
DNA
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Semancik, J. S., Morris, T. J., Weathers, L. G., Rodorf, B. F., & Kearns, D. R. (1975). Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. Virology, 63(1), 160-167. https://doi.org/10.1016/0042-6822(75)90381-5

Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. / Semancik, J. S.; Morris, Thomas J; Weathers, L. G.; Rodorf, B. F.; Kearns, D. R.

In: Virology, Vol. 63, No. 1, 01.01.1975, p. 160-167.

Research output: Contribution to journalArticle

Semancik, JS, Morris, TJ, Weathers, LG, Rodorf, BF & Kearns, DR 1975, 'Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease', Virology, vol. 63, no. 1, pp. 160-167. https://doi.org/10.1016/0042-6822(75)90381-5
Semancik, J. S. ; Morris, Thomas J ; Weathers, L. G. ; Rodorf, B. F. ; Kearns, D. R. / Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. In: Virology. 1975 ; Vol. 63, No. 1. pp. 160-167.
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