Abstract
Angiosperm systematics has progressed to the point where it is now expected that multiple, independent markers be used in phylogenetic studies. Universal primers for amplifying informative regions of the chloroplast genome are readily available, but in the faster-evolving nuclear genome it is challenging to discover priming sites that are conserved across distantly related taxa. With goals including the identification of informative markers in rosids, and perhaps other angiosperms, we screened 141 nuclear primer combinations for phylogenetic utility in two distinct groups of rosids at different taxonomic levels-Psiguria (Cucurbitaceae) and Geraniaceae. We discovered three phylogenetically informative regions in Psiguria and two in Geraniaceae, but none that were useful in both groups. Extending beyond rosids, we combined our findings with those of another recent effort testing these primer pairs in Asteraceae, Brassicaceae, and Orchidaceae. From this comparison, we identified 32 primer combinations that amplified regions in representative species of at least two of the five distantly related angiosperm families, giving some prior indication about phylogenetic usefulness of these markers in other flowering plants. This reduced set of primer pairs for amplifying low-copy nuclear markers along with a recommended experimental strategy provide a framework for identifying phylogenetically informative regions in angiosperms.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1013-1026 |
| Number of pages | 14 |
| Journal | Molecular Phylogenetics and Evolution |
| Volume | 48 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2008 |
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Keywords
- Angiosperms
- Cucurbitaceae
- Geraniaceae
- Low-copy nuclear markers
- MoBIoS
- Molecular systematics
- Phylogenetic utility
- Psiguria
- Rosids
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology
- Genetics
Cite this
Phylogenetic utility of 141 low-copy nuclear regions in taxa at different taxonomic levels in two distantly related families of rosids. / Steele, P. Roxanne; Guisinger-Bellian, Mary; Linder, C. Randal; Jansen, Robert K.
In: Molecular Phylogenetics and Evolution, Vol. 48, No. 3, 01.09.2008, p. 1013-1026.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phylogenetic utility of 141 low-copy nuclear regions in taxa at different taxonomic levels in two distantly related families of rosids
AU - Steele, P. Roxanne
AU - Guisinger-Bellian, Mary
AU - Linder, C. Randal
AU - Jansen, Robert K.
PY - 2008/9/1
Y1 - 2008/9/1
N2 - Angiosperm systematics has progressed to the point where it is now expected that multiple, independent markers be used in phylogenetic studies. Universal primers for amplifying informative regions of the chloroplast genome are readily available, but in the faster-evolving nuclear genome it is challenging to discover priming sites that are conserved across distantly related taxa. With goals including the identification of informative markers in rosids, and perhaps other angiosperms, we screened 141 nuclear primer combinations for phylogenetic utility in two distinct groups of rosids at different taxonomic levels-Psiguria (Cucurbitaceae) and Geraniaceae. We discovered three phylogenetically informative regions in Psiguria and two in Geraniaceae, but none that were useful in both groups. Extending beyond rosids, we combined our findings with those of another recent effort testing these primer pairs in Asteraceae, Brassicaceae, and Orchidaceae. From this comparison, we identified 32 primer combinations that amplified regions in representative species of at least two of the five distantly related angiosperm families, giving some prior indication about phylogenetic usefulness of these markers in other flowering plants. This reduced set of primer pairs for amplifying low-copy nuclear markers along with a recommended experimental strategy provide a framework for identifying phylogenetically informative regions in angiosperms.
AB - Angiosperm systematics has progressed to the point where it is now expected that multiple, independent markers be used in phylogenetic studies. Universal primers for amplifying informative regions of the chloroplast genome are readily available, but in the faster-evolving nuclear genome it is challenging to discover priming sites that are conserved across distantly related taxa. With goals including the identification of informative markers in rosids, and perhaps other angiosperms, we screened 141 nuclear primer combinations for phylogenetic utility in two distinct groups of rosids at different taxonomic levels-Psiguria (Cucurbitaceae) and Geraniaceae. We discovered three phylogenetically informative regions in Psiguria and two in Geraniaceae, but none that were useful in both groups. Extending beyond rosids, we combined our findings with those of another recent effort testing these primer pairs in Asteraceae, Brassicaceae, and Orchidaceae. From this comparison, we identified 32 primer combinations that amplified regions in representative species of at least two of the five distantly related angiosperm families, giving some prior indication about phylogenetic usefulness of these markers in other flowering plants. This reduced set of primer pairs for amplifying low-copy nuclear markers along with a recommended experimental strategy provide a framework for identifying phylogenetically informative regions in angiosperms.
KW - Angiosperms
KW - Cucurbitaceae
KW - Geraniaceae
KW - Low-copy nuclear markers
KW - MoBIoS
KW - Molecular systematics
KW - Phylogenetic utility
KW - Psiguria
KW - Rosids
UR - http://www.scopus.com/inward/record.url?scp=50249178528&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50249178528&partnerID=8YFLogxK
U2 - 10.1016/j.ympev.2008.05.017
DO - 10.1016/j.ympev.2008.05.017
M3 - Article
C2 - 18620872
AN - SCOPUS:50249178528
VL - 48
SP - 1013
EP - 1026
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
IS - 3
ER -