Phycodnavirus potassium ion channel proteins question the virus molecular piracy hypothesis

Kay Hamacher, Timo Greiner, Hiroyuki Ogata, James L. van Etten, Manuela Gebhardt, Luis P. Villarreal, Cristian Cosentino, Anna Moroni, Gerhard Thiel

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13 Scopus citations

Abstract

Phycodnaviruses are large dsDNA, algal-infecting viruses that encode many genes with homologs in prokaryotes and eukaryotes. Among the viral gene products are the smallest proteins known to form functional K+ channels. To determine if these viral K+ channels are the product of molecular piracy from their hosts, we compared the sequences of the K+ channel pore modules from seven phycodnaviruses to the K+ channels from Chlorella variabilis and Ectocarpus siliculosus, whose genomes have recently been sequenced. C. variabilis is the host for two of the viruses PBCV-1 and NY-2A and E. siliculosus is the host for the virus EsV-1. Systematic phylogenetic analyses consistently indicate that the viral K+ channels are not related to any lineage of the host channel homologs and that they are more closely related to each other than to their host homologs. A consensus sequence of the viral channels resembles a protein of unknown function from a proteobacterium. However, the bacterial protein lacks the consensus motif of all K+ channels and it does not form a functional channel in yeast, suggesting that the viral channels did not come from a proteobacterium. Collectively, our results indicate that the viruses did not acquire their K+ channel-encoding genes from their current algal hosts by gene transfer; thus alternative explanations are required. One possibility is that the viral genes arose from ancient organisms, which served as their hosts before the viruses developed their current host specificity. Alternatively the viral proteins could be the origin of K+ channels in algae and perhaps even all cellular organisms.

Original languageEnglish (US)
Article numbere38826
JournalPloS one
Volume7
Issue number6
DOIs
StatePublished - Jun 7 2012

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Hamacher, K., Greiner, T., Ogata, H., van Etten, J. L., Gebhardt, M., Villarreal, L. P., Cosentino, C., Moroni, A., & Thiel, G. (2012). Phycodnavirus potassium ion channel proteins question the virus molecular piracy hypothesis. PloS one, 7(6), [e38826]. https://doi.org/10.1371/journal.pone.0038826