Phosphotransferase activity in Clostridium acetobutylicum from acidogenic and solventogenic phases of growth

Robert W Hutkins, E. R. Kashket

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.

Original languageEnglish (US)
Pages (from-to)1121-1123
Number of pages3
JournalApplied and environmental microbiology
Volume51
Issue number5
StatePublished - Jan 1 1986

Fingerprint

Clostridium acetobutylicum
2-deoxyglucose
phosphotransferases (kinases)
Phosphoenolpyruvate
glucose
Phosphotransferases
phosphate
membrane
Growth
Butanols
Deoxyglucose
cells
butanol
Fructose
cell membranes
fructose
Cell Membrane
phosphates
Glucose
effect

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Phosphotransferase activity in Clostridium acetobutylicum from acidogenic and solventogenic phases of growth. / Hutkins, Robert W; Kashket, E. R.

In: Applied and environmental microbiology, Vol. 51, No. 5, 01.01.1986, p. 1121-1123.

Research output: Contribution to journalArticle

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