Phosphorylation of Vpr regulates HIV type 1 nuclear import and macrophage infection

Isabelle Agostini, Serguei Popov, Tang Hao, Jian Hua Li, Larisa Dubrovsky, Oleg Chaika, Nina Chaika, Robert E Lewis, Michael Bukrinsky

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34 Scopus citations

Abstract

Viral protein R (Vpr) of human immunodeficiency virus type 1 (HIV-1) is a small accessory protein that regulates nuclear import of the viral preintegration complex and facilitates infection of nondividing cells, such as macrophages. Studies demonstrated that a fraction of Vpr molecules is phosphorylated in the virions and in HIV-1-infected cells, but the role of phosphorylation in nuclear import activity of Vpr has not been established. We found that Vpr is phosphorylated predominantly on the serine residue in position 79, and mutations affecting Vpr phosphorylation significantly attenuated viral replication in macrophages, but not in activated T lymphocytes or cell lines. The replication defect was mapped by polymerase chain reaction analysis to the step of nuclear import. These results suggest that phosphorylation of Vpr regulates its activity in the nuclear import of the HIV-1 preintegration complex.

Original languageEnglish (US)
Pages (from-to)283-288
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume18
Issue number4
DOIs
Publication statusPublished - Jan 1 2002

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Agostini, I., Popov, S., Hao, T., Li, J. H., Dubrovsky, L., Chaika, O., ... Bukrinsky, M. (2002). Phosphorylation of Vpr regulates HIV type 1 nuclear import and macrophage infection. AIDS Research and Human Retroviruses, 18(4), 283-288. https://doi.org/10.1089/088922202753472856