Phosphorylation of the kinase suppressor of Ras by associated kinases

Deanna J. Volle, Jennifer A. Fulton, Oleg V. Chaika, Kim McDermott, Han Huang, Laurey A Steinke, Robert E Lewis

Research output: Contribution to journalArticle

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Abstract

The kinase suppressor of Ras (KSR) is a loss-of-function allele that suppresses the rough eye phenotype of activated Ras in Drosophila and the multivulval phenotype of activated Ras in Caenorhabditis elegans. The physiological role of mammalian KSR is not known. We examined the mechanisms regulating the phosphorylation of this putative kinase in mammalian cells. Wild-type mouse KSR and a mutated KSR protein predicted to create a kinase- dead protein are phosphorylated identically in intact cells and in the immune complex. Phosphopeptide sequencing identified 10 in vivo phosphorylation sites in KSR, all of which reside in the 539 noncatalytic amino terminal amino acids. Expression of the amino terminal portion of KSR alone demonstrated that it was phosphorylated in the intact cell and in an immune complex in a manner indistinguishable from that of intact KSR. These data demonstrate that the kinase domain of KSR is irrelevant to its phosphorylation state and suggest that the phosphorylation of KSR and its association with a distinct set of kinases may affect intracellular signaling.

Original languageEnglish (US)
Pages (from-to)5130-5137
Number of pages8
JournalBiochemistry
Volume38
Issue number16
DOIs
StatePublished - Apr 20 1999

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Phosphorylation
Phosphotransferases
Antigen-Antibody Complex
Phenotype
KSR-1 protein kinase
ras Proteins
Phosphopeptides
Caenorhabditis elegans
Protein Kinases
Drosophila
Alleles
Cells
Association reactions
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Volle, D. J., Fulton, J. A., Chaika, O. V., McDermott, K., Huang, H., Steinke, L. A., & Lewis, R. E. (1999). Phosphorylation of the kinase suppressor of Ras by associated kinases. Biochemistry, 38(16), 5130-5137. https://doi.org/10.1021/bi983050d

Phosphorylation of the kinase suppressor of Ras by associated kinases. / Volle, Deanna J.; Fulton, Jennifer A.; Chaika, Oleg V.; McDermott, Kim; Huang, Han; Steinke, Laurey A; Lewis, Robert E.

In: Biochemistry, Vol. 38, No. 16, 20.04.1999, p. 5130-5137.

Research output: Contribution to journalArticle

Volle, DJ, Fulton, JA, Chaika, OV, McDermott, K, Huang, H, Steinke, LA & Lewis, RE 1999, 'Phosphorylation of the kinase suppressor of Ras by associated kinases', Biochemistry, vol. 38, no. 16, pp. 5130-5137. https://doi.org/10.1021/bi983050d
Volle DJ, Fulton JA, Chaika OV, McDermott K, Huang H, Steinke LA et al. Phosphorylation of the kinase suppressor of Ras by associated kinases. Biochemistry. 1999 Apr 20;38(16):5130-5137. https://doi.org/10.1021/bi983050d
Volle, Deanna J. ; Fulton, Jennifer A. ; Chaika, Oleg V. ; McDermott, Kim ; Huang, Han ; Steinke, Laurey A ; Lewis, Robert E. / Phosphorylation of the kinase suppressor of Ras by associated kinases. In: Biochemistry. 1999 ; Vol. 38, No. 16. pp. 5130-5137.
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