Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)-ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration

Shuping Yang, Ming Ji, Lin Zhang, Yuanhong Chen, Dirk Oliver Wennmann, Joachim Kremerskothen, Jixin Dong

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In mammals, KIBRA is defined as a memory performance-associated protein. The physiological function and regulation of KIBRA in non-neuronal cells are much less understood. Recent studies have identified KIBRA as a novel regulator of the Hippo signaling pathway, which plays a critical role in tumorigenesis by inhibiting cell proliferation and promoting apoptosis. We recently reported that KIBRA is phosphorylated by the mitotic kinases Aurora and cyclin-dependent kinase 1 during mitosis. In this current study, we show that KIBRA is also phosphorylated by the ERK (extracellular signal-regulated kinases)-RSK (p90 ribosomal S6 kinases) cascade. We demonstrated that ERK1/2 phosphorylate KIBRA at Ser548 in cells as well as in vitro. Moreover, we found that RSK1/2 specifically phosphorylates KIBRA at two highly conserved sites (Thr929 and Ser947) in vitro and in cells. RSK-mediated phosphorylation is required for KIBRA binding to RSK1, but not RSK2. Surprisingly, KIBRA knockdown impaired cell migration and proliferation in breast cancer cells. By using inducible-expression cell lines, we further show that phospho-regulation of KIBRA by ERK1/2 and RSK1/2 is required for proper cell proliferation and RSK-mediated phosphorylation also modulates KIBRA's migratory activity in MDA-MB-231 breast cancer cells. Our findings uncover unexpected results and a new mechanism through which KIBRA regulates cell migration and proliferation.

Original languageEnglish (US)
Pages (from-to)343-351
Number of pages9
JournalCellular Signalling
Volume26
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Ribosomal Protein S6 Kinases
Extracellular Signal-Regulated MAP Kinases
Cell Movement
Phosphorylation
Cell Proliferation
90-kDa Ribosomal Protein S6 Kinases
Aurora Kinases
CDC2 Protein Kinase
Breast Neoplasms
Mitosis
Mammals
Carcinogenesis
Apoptosis
Cell Line
Proteins

Keywords

  • ERK1/2
  • KIBRA
  • Migration
  • Phosphorylation
  • Proliferation
  • RSK1/2

ASJC Scopus subject areas

  • Cell Biology

Cite this

Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)-ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration. / Yang, Shuping; Ji, Ming; Zhang, Lin; Chen, Yuanhong; Wennmann, Dirk Oliver; Kremerskothen, Joachim; Dong, Jixin.

In: Cellular Signalling, Vol. 26, No. 2, 01.01.2014, p. 343-351.

Research output: Contribution to journalArticle

Yang, Shuping ; Ji, Ming ; Zhang, Lin ; Chen, Yuanhong ; Wennmann, Dirk Oliver ; Kremerskothen, Joachim ; Dong, Jixin. / Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)-ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration. In: Cellular Signalling. 2014 ; Vol. 26, No. 2. pp. 343-351.
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