Phosphorylation of B-Myb regulates its transactivation potential and DNA binding

Teresa K. Johnson, Rebecca E. Schweppe, John Septer, Robert E Lewis

Research output: Contribution to journalArticle

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Abstract

The transcription factor B-Myb is a cell cycle-regulated phosphoprotein and a potent regulator of cell cycle progression. Previous studies demonstrated that B-Myb was phosphorylated at the onset of S phase, suggesting that it could be due to cyclin-dependent kinases. We identified 10 B-Myb phosphorylation sites by automated peptide radiosequencing of tryptic phosphopeptides derived from in vivo 32P-labeled B-Myb. Each B-Myb phosphorylation site contained a phosphoserine or phosphothreonine followed by a proline, suggesting that this phosphorylation is due to a proline- directed kinase. Cyclin A-Cdk2 and cyclin E-Cdk2 complexes each phosphorylated B-Myb in a cell-free system on the same sites as in intact cells. Furthermore, the ability of B-Myb to activate a reporter plasmid was enhanced by the cotransfection of cyclin A, whereas mutagenesis of the 10 identified phosphorylation sites from B-Myb blocked the effect of cyclin A coexpression. Additional analysis revealed that the effect of phosphorylation on B-Myb transactivation potential was enhanced by phosphorylation sites in its carboxyl-terminal half. One phosphorylation site (Ser581) appeared to negatively regulate DNA binding, as mutation of this site enhanced the ability of B-Myb to bind a Myb-binding sequence. These data suggest that B- Myb is a target for phosphorylation by cyclin-Cdk2 and that phosphorylation of B-Myb regulates its transcriptional activity.

Original languageEnglish (US)
Pages (from-to)36741-36749
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number51
DOIs
StatePublished - Dec 17 1999

Fingerprint

Phosphorylation
Transcriptional Activation
DNA
Cyclin A
Proline
Cell Cycle
Cells
Phosphothreonine
Phosphoserine
Phosphopeptides
Cyclin E
Mutagenesis
Cyclins
Cell-Free System
Cyclin-Dependent Kinases
Phosphoproteins
S Phase
Plasmids
Transcription Factors
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Phosphorylation of B-Myb regulates its transactivation potential and DNA binding. / Johnson, Teresa K.; Schweppe, Rebecca E.; Septer, John; Lewis, Robert E.

In: Journal of Biological Chemistry, Vol. 274, No. 51, 17.12.1999, p. 36741-36749.

Research output: Contribution to journalArticle

Johnson, Teresa K. ; Schweppe, Rebecca E. ; Septer, John ; Lewis, Robert E. / Phosphorylation of B-Myb regulates its transactivation potential and DNA binding. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 51. pp. 36741-36749.
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