Phenotypic and genotypic characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas

C. M. Araya, A. T. Alleyne, J. R. Steadman, K. M. Eskridge, D. P. Coyne

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Populations of 90 Uromyces appendiculatus isolates were collected from throughout the Americas and evaluated for virulence on 19 standard bean rust differentials, and also on 12 landraces of Phaseolus vulgaris from South and Central America. The landrace differentials represented geographical centers of bean domestication. Three groups were observed. Two groups were isolates from centers of bean domestication and a third heterogeneous group comprised isolates from countries in South and Central America. Molecular analysis using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was also conducted on these isolates. Cluster analysis of the molecular profiles showed three groups that corresponded to those obtained by virulence tests. These results show a clear differentiation of the pathogen population along similar lines as its host and suggest parallel evolution in the bean rust pathosystem.

Original languageEnglish (US)
Pages (from-to)830-836
Number of pages7
JournalPlant disease
Volume88
Issue number8
DOIs
StatePublished - Aug 2004

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Uromyces appendiculatus
domestication
Central America
landraces
Phaseolus vulgaris
beans
virulence
random amplified polymorphic DNA technique
parallel evolution
DNA-directed DNA polymerase
cluster analysis
polymerase chain reaction
pathogens
testing
bean rust

Keywords

  • Coevolution

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Phenotypic and genotypic characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas. / Araya, C. M.; Alleyne, A. T.; Steadman, J. R.; Eskridge, K. M.; Coyne, D. P.

In: Plant disease, Vol. 88, No. 8, 08.2004, p. 830-836.

Research output: Contribution to journalArticle

Araya, C. M. ; Alleyne, A. T. ; Steadman, J. R. ; Eskridge, K. M. ; Coyne, D. P. / Phenotypic and genotypic characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas. In: Plant disease. 2004 ; Vol. 88, No. 8. pp. 830-836.
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