Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and mincle

Siddappa Nagadenahalli Byrareddy, Dawn Little, Ann E. Mayne, Francois Villinger, Aftab A. Ansari

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lectin-like molecules and their receptors are cell surface molecules that have been shown to play a role in either facilitating infection or serving as transporters of HIV/SIV in vivo. The role of these lectin-like molecules in the pathogenesis of HIV/SIV infection continues to be defined. In efforts to gain further insight on the potential role of these lectin-like molecules, our laboratory generated monoclonal antibodies (mAb) against the human analogs of rhesus macaque CD200, CD200R and Mincle, since the rhesus macaques are accepted as the most reliable animal model to study human HIV infection. The characterization of the cell lineages from the blood and various tissues of rhesus macaques that express these lectin- like molecules are described herein. Among the mononuclear cells, the cells of the myeloid lineage of rhesus macaques are the predominant cell lineages that express readily detectable levels of CD200, CD200R and Mincle that is similar to the expression of Siglec-1 and Siglec-3 reported by our laboratory earlier. Subset analysis revealed that a higher frequency of the CD14+/CD16- subset from normal rhesus macaques express CD200, CD200R and Mincle. Differences in the frequencies and density of expression of these molecules by the gated population of CD14+ cells from various tissues are noted with PBMC and bone marrow expressing the highest and the mononuclear cells isolated from the colon and ileum expressing the lowest levels. While a significant frequency of pDCs and mDCs express Siglec-1/Siglec-3, a much lower frequency expresses CD200, CD200R and Mincle in PBMCs from rhesus macaques. The mAb against CD200 and CD200R but not Mincle appear to inhibit the infection of macrophage tropic SIV/SHIV in vitro. We conclude that these mAbs may have potential to be used as adjunctive therapeutic agents to control/ inhibit SIV/HIV infection.

Original languageEnglish (US)
Article numbere0140689
JournalPloS one
Volume10
Issue number10
DOIs
StatePublished - Oct 15 2015

Fingerprint

Antibody Specificity
Macaca mulatta
monoclonal antibodies
Monoclonal Antibodies
Lectins
Molecules
Sialic Acid Binding Ig-like Lectin 1
Sialic Acid Binding Ig-like Lectin 3
lectins
Cell Lineage
HIV infections
HIV Infections
cells
mononuclear leukocytes
Tissue
Tropics
Macrophages
Cell Surface Receptors
Infection
Ileum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and mincle. / Byrareddy, Siddappa Nagadenahalli; Little, Dawn; Mayne, Ann E.; Villinger, Francois; Ansari, Aftab A.

In: PloS one, Vol. 10, No. 10, e0140689, 15.10.2015.

Research output: Contribution to journalArticle

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